烟草科技2025，Vol.58Issue(11)：44-52,9.DOI:10.16135/j.issn1002-0861.2025.0151
硒与钼配施对烟草黑胫病抗性的生理影响
Physiological effects of combined application of selenium and molybdenum on tobacco's resistance to black shank
摘要
Abstract
To clarify the physiological effects of combined application of selenium(Se)and molybdenum(Mo)on tobacco's resistance to black shank,K326 was used to establish a combined application system.The effects of combined Se and Mo application on tobacco disease incidence,photosynthetic characteristics,cellular structure,antioxidant metabolism and hormone content(mass fraction)were analyzed through the inoculation of Phytophthora nicotianae.The results showed that the combined Se and Mo treatment significantly reduced the disease incidence and the black shank index,with superior effects than those of the single-element treatments.This combination also raised chlorophyll content,optimized photosynthetic parameters,and repaired chloroplast disintegration and the cell wall structural damage caused by Phytophthora nicotianae infection.By activating the actions of antioxidases[catalase(CAT),superoxide dismutase(SOD),peroxidase(POD)]and defense enzymes[nitrate reductase(NR),polyphenol oxidase(PPO)]in the plants,the productions of superoxide anion(O2-·)and hydrogen peroxide(H2O2)were regulated,inhibiting membrane lipid peroxidation reactions triggered by oxidative stress,thereby reducing the accumulation of lipid peroxidation end products such as malondialdehyde(MDA)in the plants.In the meantime,the contents of auxin(IAA),abscisic acid(ABA),jasmonic acid(JA),salicylic acid(SA),soluble proteins and soluble sugars in the plants were increased,enhancing the plant's disease resistance.Therefore,the combined application of Se and Mo regulates the contents of osmotic adjustment substances by enhancing tobacco photosynthetic,antioxidant,and hormone metabolic capabilities,thereby improving tobacco disease resistance.关键词
烟草黑胫病/硒/钼/光合作用/超微结构/抗氧化酶/激素Key words
Tobacco black shank/Selenium/Molybdenum/Photosynthesis/Ultrastructure/Antioxidase/Hormone分类
农业科学引用本文复制引用
张凯,汪呵,郭宇哲,张瑜,许自成,许嘉阳,贾玮..硒与钼配施对烟草黑胫病抗性的生理影响[J].烟草科技,2025,58(11):44-52,9.基金项目
河南农业大学青年英才项目"硒调控烟草黑胫病的抗病效应研究"(30500846). （30500846）
