空军军医大学学报2025，Vol.46Issue(11)：1415-1420,6.DOI:10.13276/j.issn.2097-1656.2025.11.003
手性纳米氧化锌的构型差异对其冻伤治疗效果的影响
Impact of configuration differences of chiral ZnO nanoparticles on its frostbite treatment effect
摘要
Abstract
Objective To investigate the structure-activity relationship between different types of chiral ZnO nanoparticles(nano-ZnO)and frostbite treatment.Methods Using zinc acetate as the zinc source and glutamic acid(Glu),valine(Val),and tyrosine(Tyr)with different chiral structures as chiral inducers,chiral nano-ZnO was prepared via the co-precipitation method.Dynamic light scattering was employed to characterize the particle size and surface charge of chiral nano-ZnO.Transmission electron microscopy was used to observe the morphology of chiral nano-ZnO.The chirality of the material was investigated by circular dichroism spectroscopy.The reactive oxygen species scavenging effects of different chiral ZnO was examined by 1,3-diphenylisobenzofuran probe.A mouse frostbite animal model was established to evaluate the frostbite therapeutic effect of chiral nano-ZnO with different chiral structures.Additionally,anti-oxidative stress capacity of chiral nano-ZnO with different chiral structures was compared with an oxygen-glucose deprivation/reperfusion(OGD/R)cell model.Results The D-Tyr-ZnO,L-Val-ZnO,L-Tyr-ZnO,D-Val-ZnO,L-Glu-ZnO,and D-Glu-ZnO constructed by the co-precipitation method had chiral structures.Different chiral designs had a significant impact on the particle size,surface charge,and antioxidant capacity of nano-ZnO.There were notable differences in the therapeutic effects of chiral nano-ZnO with different chiral structures in frostbite animal model,especially L-Glu-ZnO showed better efficacy than those with other chiral structures(P＜0.05).In the OGD/R cell model,L-Glu-ZnO could significantly inhibit the cellular oxidative stress level(P＜0.05).Conclusion The chiral structure exerts an influence on the frostbite treatment efficacy of nano-ZnO.L-Glu-ZnO demonstrates promising potential for clinical applications.关键词
氧化锌/手性/冻伤/纳米医学/抗氧化应激/氨基酸/构效关系/L-谷氨酸氧化锌Key words
zinc oxide/chirality/frostbite/nanomedicine/anti-oxidative stress/amino acids/structure-activity relationship/L-Glu-ZnO分类
医药卫生引用本文复制引用
王超丽,乔友备,高豪,孙淳,翟媛,杨长斌,陈鹏..手性纳米氧化锌的构型差异对其冻伤治疗效果的影响[J].空军军医大学学报,2025,46(11):1415-1420,6.基金项目
国家自然科学基金面上项目(82173772) （82173772）
陕西省重点研发计划项目(2022ZDLSF05-06) （2022ZDLSF05-06）
