空军军医大学学报2025，Vol.46Issue(11)：1425-1432,8.DOI:10.13276/j.issn.2097-1656.2025.11.005
T7多肽修饰超小聚多巴胺纳米粒对创伤性脑损伤的治疗作用
Therapeutic effects of T7 peptide-modified ultra-small polydopamine nanoparticles on traumatic brain injury
摘要
Abstract
Objective To fabricate T7 peptide-modified ultra-small polydopamine nanoparticles(T7-UPDA)with the ability to target brain capillary endothelial cells bEnd3 and BV2 microglia,efficiently scavenge reactive oxygen species(ROS),and promote the polarization of BV2 cells toward the M2 phenotype,thereby offering a novel therapeutic strategy for traumatic brain injury(TBI).Methods The in vitro biocompatibility of T7-UPDA was evaluated through hemolysis assays and cytotoxicity experiments.The particle size,zeta potential,and stability of T7-UPDA were characterized using a laser particle size analyzer.The uptake efficiency of T7-UPDA by bEnd3 cells and scratched BV2 cells,its ability to scavenge intracellular ROS in scratched BV2 cells,and its effect on BV2 cell polarization were detected via flow cytometry.Results T7-UPDA exhibited a particle size of approximately(35.1±0.8)nm with a spherical nanoparticle morphology,and no significant change in particle size was observed in 7 d.T7-UPDA demonstrated good biocompatibility in vitro.It effectively targeted both bEnd3 cells and scratched BV2 cells,efficiently scavenged ROS in scratched BV2 cells,and promoted the polarization of BV2 cells toward the M2 phenotype.Conclusion T7-UDPA exhibits the ability to target bEnd3 cells and scratched BV2 cells.By scavenging ROS and reprogramming the polarization state of microglia,it synergistically exerts antioxidant and anti-inflammatory effects,offering new insights for developing nanotherapeutic strategies against secondary brain injury.关键词
超小聚多巴胺纳米粒/T7肽/创伤性脑损伤/活性氧清除/BV2细胞极化/脑靶向/递药系统/生物相容性Key words
ultra-small polydopamine nanoparticles/T7 peptide/traumatic brain injury/reactive oxygen species scavenging/BV2 cell polarization/brain targeting/drug delivery system/biocompatibility分类
特种医学引用本文复制引用
秦佳乐,伊春林,李晗,崔好,宫文帅,吴红燕,周四元,刘道洲..T7多肽修饰超小聚多巴胺纳米粒对创伤性脑损伤的治疗作用[J].空军军医大学学报,2025,46(11):1425-1432,8.基金项目
陕西省中医药管理局项目(SZY-KJCYC-2025-ZY-013) （SZY-KJCYC-2025-ZY-013）
陕西省重点研发计划一般项目(2023-YBSF-221) （2023-YBSF-221）
