中国草食动物科学2025，Vol.45Issue(6)：20-25,6.DOI:10.3969/j.issn.2095-3887.2025.00.026
基于转录组测序筛选与母驼卵巢发育相关候选miRNA
Screening of Candidate miRNA Related to Ovary Development in Female Camels Based on Transcriptome Sequencing
摘要
Abstract
In order to screen miRNA related to ovarian development in Bactrian camels,6 healthy non-pregnant Bac-trian camels with similar age and body weight were selected as the study subjects and divided into 2 groups(reprodu-tive season group and non-reprodutive season group)according to the test requirements.Transcriptome sequencing was used to analyze miRNA in ovarian tissues of Bactrian camel,and the differentially expressed miRNAs were screened.The GO function and KEGG pathway enrichment of differentially expressed genes were analyzed,and mi-RNAs related to ovarian development were screened.Four differentially expressed miRNAs were randomly selected for verification.The results showed that 741 miRNAs were expressed in both the reproductive and non-reproductive sea-sons,58 miRNAs were expressed specifically in the reproductive season,and 60 miRNAs were expressed specifically in the non-reproductive season.47 differentially expressed miRNAs were identified by screening.Compared with non-reproductive season,26 miRNAs were significantly up-regulated and 21 miRNAs were down-regulated in the ovary during reproductive season.KEGG enrichment analysis showed that miRNA target genes were mainly enriched in the signaling pathways related to ovarian development,such as MAPK,PI3K-Akt,AMPK,Hippo,Rap1,p53,and Wnt.Four differentially expressed miRNAs(miR-10b,miR-361,miR-196a,and miR-345-5p)participating in the above signaling pathways were selected for qRT-PCR verification.The results showed that miR-10b and miR-361 were sig-nificantly up-regulated(P<0.05),miR-196a was significantly up-regulated(P<0.01),and miR-345-5p was signifi-cantly down-regulated(P<0.01)in the ovaries of female camels during the reproductive season,which were consistent with those of miRNA sequencing.These results suggest that miRNA plays an important role in ovarian development of Bactrian camels.关键词
双峰驼/卵巢发育/miRNA/转录组测序/繁殖季节与非繁殖季节Key words
Bactrian camel/ovarian development/miRNA/transcriptome sequencing/reproductive and non-reproductive seasons分类
农业科技引用本文复制引用
娜哈雅,赫必太,张永昌,娜仁花,谢望为,刘鹤洁,吉木斯,士惠杰,张馨娜,宝音吉日嘎拉,张靖,昂格乐玛..基于转录组测序筛选与母驼卵巢发育相关候选miRNA[J].中国草食动物科学,2025,45(6):20-25,6.基金项目
内蒙古农业大学动物科学学院高水平成果培育专项项目(CG202403,BZX202213) （CG202403,BZX202213）
