中国动物检疫2025，Vol.42Issue(11)：64-70,7.DOI:10.3969/j.issn.1005-944X.2025.11.011
细菌总RNA提取方法研究进展
Advances in the Methods for Total Bacteria RNA Isolation
摘要
Abstract
Ribonucleic acid(RNA),as a crucial genetic material in bacteria,not only participates in cellular metabolic processes,but also plays an important role in bacterial gene expression and functional regulation.Successful isolation of RNA is a key for downstream molecular biology research,although isolation of high-quality RNA is a challenge due to its unstable structure and ubiquitous presence of ribonucleases(RNases).To this end,Trizol,enzymatic lysis,cetyltrimethylammonium bromide(CTAB),sodium dodecyl sulfate-hot phenol(SDS hot-phenol)and other bacteria RNA isolation methods were developed,but prone to different application scenarios due to their differences in terms of time consumption,cost,RNA output and purity,operational complexity and applicable bacterial species.Here,the characteristics of common bacteria RNA isolation methods,problems found during isolation,and application scenarios of all the methods were reviewed,aiming to provide a reference for bacteria RNA isolation.关键词
细菌RNA/提取方法/化学变性法/物理裂解法/试剂盒法Key words
bacteria RNA/isolation method/chemical denaturation method/physical lysis method/kit-based method分类
生物学引用本文复制引用
苑慧欣,孙淑芳,商营利,樊晓旭,靳继惠,刘蒙达,张皓博,亓菲,金琳,焉鑫,南文龙,孙世雄..细菌总RNA提取方法研究进展[J].中国动物检疫,2025,42(11):64-70,7.基金项目
"十四五"国家重点研发计划项目(2022YFD1800701) （2022YFD1800701）
国家现代农业(奶牛)产业技术体系建设项目(CARS-36) （奶牛）
山东省牛产业技术体系项目(SDAIT-09-06) （SDAIT-09-06）
山东省重点研发计划(重大科技创新工程)项目(2024CXGC010910) （重大科技创新工程）
