中国动物检疫2025，Vol.42Issue(11)：71-76,6.DOI:10.3969/j.issn.1005-944X.2025.11.012
沙门氏菌SYBR Green Ⅰ实时荧光PCR检测方法的建立
Development of a SYBR Green Ⅰ Real-time Fluorescence PCR Assay for Salmonella
摘要
Abstract
Salmonellosis has posed a threat to human health and development of livestock industry as a common zoonotic disease.In order to specifically and sensitively detect Salmonella,a SYBR Green Ⅰ real-time fluorescence PCR assay was developed through optimizing reaction system and conditions of specific primers that were designed based on the conserved region of fimY gene of Salmonella,and verified for its specificity,sensitivity and repeatability.The results showed that,by the established method,there was no cross-reaction with other pathogens,the minimum limit of detection was 4.47×101 copies/μL,and both the coefficients of variation(CVs)of intra-and inter-assay tests were less than 1.5％,and the coincidence rate with conventional PCR was 97.78％for clinical sample testing.In conclusion,the established method presented high specificity,sensitivity and repeatability,and could be used for epidemiological investigation and screening of Salmonellosis as a technical tool.关键词
沙门氏菌/fimY基因/实时荧光PCRKey words
Salmonella/fimY gene/real-time fluorescence quantitative PCR分类
畜牧业引用本文复制引用
张晓倩,靳木子,王瑞,赵治国,陈林军,郝静云,焦迪,延涵,崔强,王海艳,罗小平,李军燕..沙门氏菌SYBR Green Ⅰ实时荧光PCR检测方法的建立[J].中国动物检疫,2025,42(11):71-76,6.基金项目
内蒙古自治区重点研发和成果转化计划项目(2023YFDZ0048) （2023YFDZ0048）
中央引导地方科技发展资金项目(2024ZY0156) （2024ZY0156）
鄂尔多斯市国家可持续发展议程创新示范区建设(含厅市会商、科技突围工程)科技支撑项目(KCX2024016) （含厅市会商、科技突围工程）
鄂尔多斯市科研创新团队建设专项B类团队(二类团队)项目(BR251303) （二类团队）
国家乳业技术创新中心项目(2023-JSGG-5) （2023-JSGG-5）
