中国动物检疫2025，Vol.42Issue(11)：97-101,114,6.DOI:10.3969/j.issn.1005-944X.2025.11.016
4种常见布鲁氏菌抗体检测方法的评价
Evaluation on 4 Common Detection Methods for Brucella Antibodies
摘要
Abstract
In order to compare the performance of single and vertical testing(primary screening and confirmation)of common detection methods for Brucella antibodies,including Rose Bengal plate agglutination test(RBT),standard-tube agglutination test(SAT),indirect ELISA(iELISA)and competitive ELISA(cELISA),Brucella antibody-positive sera from cattle and sheep,as well as 275 clinical serum samples from these animals were singly and vertically tested by these methods to assess their analytical sensitivity,analytical specificity,positive detection rate and concordance rate.The results showed that the minimum detection limits of RBT,SAT,iELISA and cELISA for bovine sera were 25.0,100.0,3.1 and 3.1 IU/mL,respectively,and for ovine sera were 25.0,50.0,50.0 and 12.5 IU/mL,respectively;the overall positive detection rates for clinical serum samples by the 4 single methods were ranked as cELISA(70.91％)＞iELISA(66.55％)＞RBT(39.64％)＞SAT(36.00％);and iELISA screening+cELISA confirmation(61.09％)＞RBT screening+cELISA confirmation(39.64％)＞iELISA screening+SAT confirmation(36.00％)＞RBT screening+SAT confirmation(34.55％)by the vertical method;for the single methods,the consistency between agglutination methods(RBT and SAT)as well as that between ELISA methods(iELISA and cELISA)were very strong and high,respectively;for vertical methods,the consistency using agglutination methods for screening or confirmation was very strong,and that between above-mentioned combination and iELISA screening+cELISA confirmation was moderate.In conclusion,the sensitivity and concordance of the 4 methods were different when they were used separately or jointly,requiring to select a suitable method as appropriate.关键词
布鲁氏菌病/ELISA/虎红平板凝集/试管凝集/垂直检测Key words
brucellosis/ELISA/RBT/SAT/vertical detection分类
畜牧业引用本文复制引用
刘颖昳,苗清新,徐琦,毕一鸣,司国辉,郝玉欣,郄帅,顾小雪,戴维凝..4种常见布鲁氏菌抗体检测方法的评价[J].中国动物检疫,2025,42(11):97-101,114,6.基金项目
国家重点研发计划项目(2022YFC2304005) （2022YFC2304005）
