局解手术学杂志2025，Vol.34Issue(11)：933-937,5.DOI:10.11659/jjssx.11E024069
丙泊酚对脓毒症大鼠急性肾损伤的保护作用及机制
Protective effect and mechanism of propofol on acute kidney injury in septic rats
摘要
Abstract
Objective To explore the protective effect and potential mechanism of propofol on acute kidney injury in septic rats.Methods A total of 84 adult SD rats were randomly divided into the sham surgery group,the model group and the treatment group.The sham surgery group only opened and closed the abdomen,while the model group underwent cecal ligation and perforation for modeling.In the treatment group,propofol(60 mg/kg)was intraperitoneally injected 30 minutes before modeling,and the same dose of propofol was intraperitoneally injected again 6 hours after modeling.The renal function and pathological changes of rats in each group were observed,24-hour survival status of rats in each group were recorded,and the 24-hour survival rate was calculated.A sepsis model was established in vitro by stimulating human renal tubular epithelial cell line with lipopolysaccharide(LPS),and the changes of apoptosis and oxidative stress of renal tubular epithelial cells were observed.The effects of propofol on oxidative stress and apoptosis of renal tubular epithelial cells stimulated by LPS were analyzed.Results The renal function of sepsis rats was decreased,the renal tissue structure was damaged,the 24-hour survival rate of rats was decreased significantly and the survival time was shortened.Propofol could effectively improve renal function,reduce renal Paller score,increase survival rate and prolong survival time in sepsis rats.LPS could induce apoptosis and oxidative stress of renal tubular epithelial cells,while propofol could inhibit oxidative stress and apoptosis of renal tubular epithelial cells.Conclusion Propofol can effectively alleviate acute kidney injury in septic rats,and its mechanism is related to the inhibition of apoptosis and oxidative stress of renal tubular epithelial cells.关键词
丙泊酚/脓毒症/大鼠/急性肾损伤/细胞凋亡/氧化应激Key words
propofol/sepsis/rats/acute kidney injury/cell apoptosis/oxidative stress分类
临床医学引用本文复制引用
余沛,张濒丹..丙泊酚对脓毒症大鼠急性肾损伤的保护作用及机制[J].局解手术学杂志,2025,34(11):933-937,5.基金项目
国家自然科学基金项目(82300561) （82300561）
重庆市自然科学基金面上项目(CSTB2023NSCQ-MSX0713) （CSTB2023NSCQ-MSX0713）
