局解手术学杂志2025，Vol.34Issue(11)：938-943,6.DOI:10.11659/jjssx.10E024001
长链非编码RNA FGD5-AS1对垂体瘤的影响及其机制研究
Study on the effect and mechanism of long non-coding RNA FGD5-AS1 on pituitary adenoma
摘要
Abstract
Objective To investigate the effects of long non-coding RNA FGD5-AS1 on the proliferation,migration,and invasion of pituitary adenoma(PA)cells,and to analyze its potential mechanism of action.Methods Human PA cell lines HPAs,RC-4BC,HP75,and human astrocyte cell line NHA were cultured in vitro.The expression levels of FGD5-AS1 and miR-15a in the above cell lines were detected by RT-PCR.HP75 cells in the logarithmic growth phase were randomly divided into the silencing group and the negative control group.The silencing group was transfected with shRNA-FGD5-AS1,while the negative control group was transfected with shRNA-NC.The expression levels of FGD5-AS1 and miR-15a in the two groups of cells were detected by RT-PCR.The proliferation,migration and invasion abilities of the two groups of cells were determined by CCK-8 assay,wound healing assay,and Transwell assay.The expression of proteins related to the Wnt/β-catenin signaling pathway in the two groups of cells was detected by Western blot.The targeting relationship between FGD5-AS1 and miR-15a was verified by dual-luciferase reporter gene assay.Results Compared with the NHA cell,the expression level of FGD5-AS1 was significantly increased in the HPAs,RC-4BC,and HP75 cells((P<0.05),whereas the expression level of miR-15a was significantly decreased(P<0.05).Compared with the negative control group,the expression level of FGD5-AS1 was decreased(P<0.05),the expression level of miR-15a was increased(P<0.05),the OD value was decreased(P<0.05),the migration and invasion abilities of cells were reduced(P<0.05),and the expression of Wnt3a and β-catenin proteins was decreased in the silencing group of HP75 cells(P<0.05).FGD5-AS1 could specifically bind to miR-15a,leading to a decrease in cell luciferase activity(P<0.05).Conclusion FGD5-AS1 is overexpressed in PA cells,and silencing FGD5-AS1 can inhibit the proliferation,migration,and invasion of PA cells,and the mechanism is related to its targeted regulation of miR-15a.关键词
FGD5-AS1/miR-15a/垂体瘤/细胞增殖/细胞迁移/细胞侵袭/Wnt/β-catenin信号通路Key words
FGD5-AS1/miR-15a/pituitary adenoma/cell proliferation/cell migration/cell invasion/Wnt/β-catenin signaling pathway分类
临床医学引用本文复制引用
韩晓正,任洪波,贺龙,宋志远,牛国栋..长链非编码RNA FGD5-AS1对垂体瘤的影响及其机制研究[J].局解手术学杂志,2025,34(11):938-943,6.基金项目
河北省医学科学研究课题计划项目(20200194) （20200194）
