中国康复2025，Vol.40Issue(11)：643-649,7.DOI:10.3870/zgkf.2025.11.001
高压氧联合跑台运动对脑瘫幼鼠内质网应激、神经元树突棘发育及JAK/STAT信号通路的影响
Effects of hyperbaric oxygen combined with treadmill exercise on endoplasmic reticulum stress,neuronal dendritic spine development and JAK/STAT signaling pathway in young cerebral palsy rats
摘要
Abstract
Objective:To study the effects of hyperbaric oxygen combined with treadmill running on endoplasmic reticulum stress,neuronal dendritic spine development and JAK/STAT signaling pathway in young cerebral palsy rats.Methods:In total,64 young rats were randomly divided into N group(normal control group),M group(mod-el group).H group(model+hyperbaric oxygen),R group(model+treadmill exercise),U group(model+hyper-baric oxygen+treadmill exercise)(12 in N group,and 13 in the rest groups).The model of ischemic hypoxic cere-bral palsy was established except for N group.The behavioral changes of young mice in each group were detected u-sing the tactile stimulation test,and the time taken to tear off the tape was recorded.Serum levels of tumor necrosis factor α(TNF-α),interleukin 1β(IL-1β)and IL-10 were detected by ELISA.Endoplasmic reticulum stress indexes[including the protein expression of protein kinase-like endoplasmic reticulum kinase(PERK)and cysteine aspartic protease 12(Caspase-12)]were analyzed by immuno-fluorescence staining.The dendrite density of apical dendrite and basal dendrite was analyzed by staining with Golgi staining kit.Morphological changes of brain tissue and neuronal injury were observed by HE stai-ning.The expression level of JAK/STAT protein was detected by Western blotting.Results:The time of tearing off the tape was longer,and IL-10 level was lower in M group,H group,R group and U group than those in N group(P＜0.05).The time of tearing off the tape in H group,R group and U group was shorter than that in M group M,and that in U group was the shortest(P＜0.05).Compared with N group,the expression levels of serum TNF-α,IL-1β,brain PERK,Caspase-12,P-JAK2 and P-Stat3 protein in M,H,R and U groups were increased,and IL-10,the density of basal dendrite spines in CA region was decreased(P＜0.05).The levels of TNF-α,IL-1β,PERK,Caspase-12,P-JAK2 and P-Stat3 in H,R and U groups were decreased,the lowest in U group,IL-10,the density of basal dendrite spines in CA region was increased,and the highest in U group(P＜0.05).There were no significant differences in JAK2 and STAT3 levels and apical dendrite spine density in CA region among all groups.The cell morphology of H,R and U groups was improved,the cell staining became shallow,the inflammatory infiltration was reduced,and the nerve fiber tangles were alleviated,among which the cell morphology of U group showed the most obvious improvement.Compared with N group,the number of normal nerve cells in M,H,R,and U groups decreased(P＜0.05).Compared with M group,the number of normal nerve cells in H,R,and U groups increased,with U group having the greatest number(P＜0.05).Conclusion:Hyperbaric oxygen and treadmill exercise can improve the endoplasmic reticulum stress and the development of neuronal dendritic spine,inhibit the activation of JAK/STAT signaling pathway,and the combined effect is better.关键词
高压氧/跑台运动/脑瘫/内质网应激/神经元树突棘发育Key words
hyperbaric oxygen/treadmill exercise/cerebral palsy/endoplasmic reticulum stress/neuronal den-dritic spines development分类
医药卫生引用本文复制引用
李晓洁,徐玲玲,郑苏,赵峰..高压氧联合跑台运动对脑瘫幼鼠内质网应激、神经元树突棘发育及JAK/STAT信号通路的影响[J].中国康复,2025,40(11):643-649,7.基金项目
2021-2022年度湖北省卫健委中医药科研立项项目(青年人才项目)(ZY2021Q015) （青年人才项目）
