中国临床药理学杂志2025，Vol.41Issue(18)：2635-2638,4.DOI:10.13699/j.cnki.1001-6821.2025.18.011
一株携带特殊质粒的万古霉素耐药屎肠球菌基因组特征分析
Genomic characterization of a vancomycin-resistant Enterococcus faecium strain carrying a special plasmid
苏博 1郑波1
作者信息
- 1. 北京大学第一医院临床药理研究所,北京 100034
- 折叠
摘要
Abstract
Objective To sequence a vancomycin-resistant Enterococcus faecium and analyze the vanM gene cluster carried on its plasmid as well as the genes conferring advantage in nutrient utilization.Method The broth microdilution method was used to perform drug susceptibility tests on strain 15P371 and its transconjugant J15P371.Subsequently,whole-genome sequencing was conducted using Nanopore technology for both strains,followed by analysis of the resistant plasmid sequences.Bacterial growth experiments were carried out to verify the advantage in intestinal nutrient utilization.Results Strain 15P371 is a vancomycin-resistant strain carrying 6 copies of the vanM gene cluster.Its transconjugant J15P371 can tolerate a higher concentration of vancomycin but only harbors 3 copies of the vanM gene cluster.Genes conferring advantage in intestinal N-acetylgalactosamine utilization are present on the resistant plasmid.Conclusion In vanM-type vancomycin-resistant strain,the copy number of resistance genes is variable during conjugative transfer.The plasmid carrying genes with advantage in N-acetylgalactosamine utilization confer growth advantage to the host bacteria.关键词
万古霉素/屎肠球菌/vanM基因簇/N-乙酰半乳糖胺利用基因Key words
vancomycin/Enterococcus faecium/vanM gene cluster/N-acetylgalactosamine utilization genes分类
药学引用本文复制引用
苏博,郑波..一株携带特殊质粒的万古霉素耐药屎肠球菌基因组特征分析[J].中国临床药理学杂志,2025,41(18):2635-2638,4.
