中国免疫学杂志2025，Vol.41Issue(11)：2573-2577,5.DOI:10.3969/j.issn.1000-484X.2025.11.003
解脲脲原体LAMPs通过正向调控巨噬细胞M1极化抑制铁死亡机制研究
Ureaplasma spp.LAMPs inhibits ferroptosis by positively regulation of macrophage M1 polarization
摘要
Abstract
Objective:To investigate the effect of Ureaplasma spp.lipid-associated membrane proteins(LAMPs)on macro-phage M1 polarization,and clarify how LAMPs resist to ferroptosis by regulating macrophage M1 polarization.The overall goal is to contribute to a better understanding of pathogenesis of non-gonococcal urethritis caused by Ureaplasma spp.Methods:ELISA,qPCR and flow cytometry were used to analyze the effect of LAMPs on M1 polarization of macrophages;Western blot to detect how LAMPs stimulates NF-κB pathway during M1 polarization of macrophages;gene interference technology was used to elucidate how LAMPs affects macrophage M1 polarization through regulating NF-κB pathway;CCK8 and Western blot were used to find out how LAMPs affects macrophage ferroptosis by regulating NF-κB pathway.Results:ELISA,qPCR and flow cytometry results showed that LAMPs promoted macrophage polarization to M1;Western blot results showed that LAMPs increased levels of p-p65 in macrophages;interfering with p65 expression could inhibit M1 polarization process of macrophages that mediated by LAMPs,and inhibition of macrophage ferroptosis that mediated by LAMPs could be significantly reversed.Conclusion:LAMPs inhibits ferroptosis by accelerating macrophage M1 po-larization,which is one of the important pathogenic factors of non-gonococcal urethritis caused by Ureaplasma spp.关键词
解脲脲原体脂结合膜蛋白/巨噬细胞M1极化/铁死亡/NF-κBKey words
Ureaplasma spp.LAMPs/Macrophages M1 polarization/Ferroptosis/NF-κB分类
医药卫生引用本文复制引用
毕敬磊,田茂瑾,赵培庆,张荣葵..解脲脲原体LAMPs通过正向调控巨噬细胞M1极化抑制铁死亡机制研究[J].中国免疫学杂志,2025,41(11):2573-2577,5.基金项目
国家自然科学基金项目(12304241,81972002) （12304241,81972002）
山东省自然科学基金项目(ZR2023QC168). （ZR2023QC168）
