中国免疫学杂志2025，Vol.41Issue(11)：2591-2595,5.DOI:10.3969/j.issn.1000-484X.2025.11.006
TNFAIP8调节Treg细胞对肾移植中免疫耐受的影响
Effect of TNFAIP8 on immune tolerance in kidney transplantation by regulating Treg cells
摘要
Abstract
Objective:To explore the mechanism of tumor necrosis factor-α-inducible protein-8(TNFAIP8)regulating CD4+CD25+FOXP3+Treg cells involved in immune tolerance of kidney transplantation model.Methods:Wistar rats were used as recipients of kidney transplantation and divided into control,model,model+TNFAIP8,model+TNFAIP8+CD25 groups,with 12 rats in each group.SD rats were used as kidney donors,and the left kidney was transplanted into Wistar rats.Lentivirus-TNFAIP8(300 µg/kg)was injected into the tail vein to overexpress TNFAIP8.CD25 antibody at 10 mg/kg was injected into the tail vein to inhibit the differen-tiation of Treg.Renal tissue injury,renal function,Treg cell ratio,protein levels of TNFAIP8 and FOXP3 were compared.Results:Compared with control group,BUN[(23.85±20.68)mmol/L]and Scr[(112.49±10.02)µmol/L]in model group were increased,while proportion of Treg cells[(3.01±0.32)%],protein levels of TNFAIP8 and FOXP3 were decreased(P<0.05).BUN[(11.74±11.04)mmol/L]and Scr[(65.56±6.15)µmol/L]in model+TNFAIP8 group were lower than those in model group,Treg level[(6.74±0.68)%],TNFAIP8 and FOXP3 protein levels were higher than those in model group(P<0.05).BUN[(20.26±2.19)mmol/L]and Scr[(100.82±8.27)µmol/L]in model+TNFAIP8+CD25 group were higher than those in model+TNFAIP8 group,while the levels of Treg[(3.34±0.36)%]and FOXP3 protein were lower than those in model+TNFAIP8 group(P<0.05).Conclusion:TNFAIP8 involved in immune tolerance in kidney transplantation by regulating CD4+CD25+FOXP3+Treg cells.关键词
肾移植/TNFAIP8/Treg/免疫耐受Key words
Kidney transplantation/TNFAIP8/Treg/Immune tolerance分类
医药卫生引用本文复制引用
王振璞,房军..TNFAIP8调节Treg细胞对肾移植中免疫耐受的影响[J].中国免疫学杂志,2025,41(11):2591-2595,5.基金项目
河南省科技计划项目(212102310668). （212102310668）
