中国免疫学杂志2025，Vol.41Issue(11)：2596-2601,中插1,7.DOI:10.3969/j.issn.1000-484X.2025.11.007
蛋氨酸脑啡肽上调巨噬细胞调理性受体抑制流感病毒感染的研究
Research of upregulation of macrophage opsonizing receptors by methionine enkephalin in inhibiting influenza virus infection
摘要
Abstract
Objective:To investigate immunomodulatory effects of methionine enkephalin(MENK)on macrophages,and to explore effect of opsonizing receptors in anti-influenza virus infection of macrophages.Methods:Potential targets for antiviral effects of MENK on macrophages were explored by network pharmacology.Proteomics analysis was used to identify differentially expressed pro-teins(DEPs)in macrophages of MENK-PR8 and PR8 groups.DEPs were analyzed by bioinformatics,and key factors were verified by qPCR and Western blot.Results:MENK had 85 intersection targets with macrophages and influenza viruses,of which 7 were related to phagosome pathway(mmu04145).A total of 215 DEPs were identified by mass spectrometry,which were highly enriched in phago-some(mmu04145)and interaction of viral proteins with cytokines and cytokine receptors(mmu04061)pathways.qPCR and Western blot showed that Fc gamma receptor(FcγR)and complement receptor(CR3)related to phagosome were highly expressed.Conclu-sion:MENK enhances function of phagocytosis and killing virus by upregulating opsonizing receptors via opioid receptor,suggesting that MENK can serve as an immune modulator or a novel preventive drug for influenza viruses.关键词
蛋氨酸脑啡肽/巨噬细胞/流感病毒/调理性受体/蛋白质谱分析Key words
MENK/Macrophage/Influenza virus/Opsonizing receptors/Proteomics analysis分类
医药卫生引用本文复制引用
魏刚,付纹瑞,陈月,王晓萌,赵园龙,田晶..蛋氨酸脑啡肽上调巨噬细胞调理性受体抑制流感病毒感染的研究[J].中国免疫学杂志,2025,41(11):2596-2601,中插1,7.基金项目
辽宁省博士科研启动基金计划项目(2020-BS-246) （2020-BS-246）
辽宁省教育厅基本科研面上项目(LJKMZ20221239). （LJKMZ20221239）
