中国免疫学杂志2025，Vol.41Issue(11)：2602-2606,5.DOI:10.3969/j.issn.1000-484X.2025.11.008
幽门螺杆菌介导的中性粒细胞凋亡障碍机制研究
Mechanism of Helicobacter pylori-mediated neutrophil apoptosis disorder
摘要
Abstract
Objective:To investigate molecular mechanism of Helicobacter pylori(H.pylori)-mediated neutrophil apoptosis disorder.Methods:Neutrophils extracted from peripheral blood of healthy adults were used as research subjects,and H.pylori stan-dard strain NCTC11637 was added to culture system to construct a model of in vitro infection,which was divided into control group(with PBS or medium treatment),H.pylori group(H.pylori co-cultivated with neutrophils at ratio of 10∶1).After a certain time of in-fection,apoptosis-related molecules of neutrophils were detected by flow cytometry,ELISA and Western blot,respectively.Results:Cytokines IL-1β,IL-6 and TNF-α were secreted significantly higher in H.pylori group than control group,apoptosis rate was signifi-cantly lower than control group,apoptosis-related proteins Caspase-3 and Caspase-8 were higher than control group,but their activa-tion level were lower than control group.Conclusion:H.pylori infection may prolong neutrophil survival by inhibiting apoptotic pro-cess of neutrophils through inhibiting activation of Caspase-3/8.关键词
幽门螺杆菌/中性粒细胞/细胞凋亡Key words
Helicobacter pylori/Neutrophils/Apoptosis分类
医药卫生引用本文复制引用
毕红梅,许畅,时小淋,李丹,刘鹏..幽门螺杆菌介导的中性粒细胞凋亡障碍机制研究[J].中国免疫学杂志,2025,41(11):2602-2606,5.基金项目
威海市立医院卓越精英项目(FH2022JZ01). （FH2022JZ01）
