中国免疫学杂志2025，Vol.41Issue(11)：2607-2612,2618,7.DOI:10.3969/j.issn.1000-484X.2025.11.009
橄榄苦苷调节Hippo-YAP信号通路对OGD/R诱导的神经元损伤的修复作用
Repair effect of oleuropein on OGD/R-induced neuronal damage by regulating Hippo-YAP signaling pathway
摘要
Abstract
Objective:To investigate the effects of oleuropein(OE)on oxygen glucose deprivation/reperfusion(OGD/R)in-duced neuronal damage and Hippo-Yes associated protein(YAP)signaling pathway.Methods:Neurons were grouped into Control group,OGD/R group,OE-L group,OE-M group,OE-H group and OE-H+YAP inhibitor(VP)group;detection of cell activity;cell proliferation and apoptosis were detected in each group;the levels of inflammatory factors and oxidative stress were detected;the ex-pression levels of Cyclin D1,cell cycle negative regulator(P21),B cell lymphoma 2(Bcl-2),Bcl-2 associated X protein(Bax),YAP and transcription coactivator with PDZ-binding motif(TAZ)were detected.Results:In OGD/R group,deep staining of neuronal cell nuclei,EdU positive rate,Cyclin D1,Bcl-2,YAP and TAZ expressions were decreased,P21,TNF-α,IL-6,MDA,SOD,apop-tosis rate and Bax expression were increased(P<0.05).The cell nucleus was gradually restored to normal by OE,the blue staining was gradually lightest,the EdU positive rate,Cyclin D1,Bcl-2,YAP and TAZ levels were increased,and the expressions of P21,TNF-α,IL-6,MDA,SOD,apoptosis rate and Bax were decreased(P<0.05);VP reverse protective effect of OE on OGD/R-induced neuronal injury.Conclusion:OE can improve OGD/R-induced neuronal damage by activating Hippo/YAP signaling pathway.关键词
橄榄苦苷/Hippo-Yes相关蛋白信号通路/氧糖剥夺/再灌注/神经元损伤Key words
Oleuropein/Hippo-Yes associated protein signaling pathway/Oxygen glucose deprivation/reperfusion/Neuron damage分类
临床医学引用本文复制引用
邓艳玲,吴智刚,肖茜,张科..橄榄苦苷调节Hippo-YAP信号通路对OGD/R诱导的神经元损伤的修复作用[J].中国免疫学杂志,2025,41(11):2607-2612,2618,7.基金项目
2021年度海南省卫生健康行业科研项目(21A200320). （21A200320）
