中国免疫学杂志2025，Vol.41Issue(11)：2613-2618,6.DOI:10.3969/j.issn.1000-484X.2025.11.010
厄贝沙坦调节cGAS-STING信号通路对缺氧复氧诱导的心肌细胞焦亡的影响
Effect of irbesartan on hypoxia reoxygenation induced myocardial cell pyroptosis by regulating cGAS-STING signaling pathway
摘要
Abstract
Objective:To investigate the effect of irbesartan on hypoxia reoxygenation(HR)induced myocardial cell pyropto-sis by regulating the cGAS-STING signaling pathway.Methods:H9c2 myocardial cells were cultured in vitro and grouped into control group,HR group,low-dose irbesartan group,high-dose irbesartan group,high-dose irbesartan+RocA(cGAS-STING signaling path-way activator)group,and RU.521(cGAS-STING signaling pathway inhibitor)group.Cell viability was detected by CCK-8;ELISA was applied to detect levels of lactate dehydrogenase(LDH),IL-6,IL-18 and IL-1β;propidium iodide(PI)staining method was applied to detect the formation of cell membrane pores;RT-qPCR method was applied to detect the expressions of cGAS and STING mRNA in cells;Western blot method was applied to detect the expressions of NLRP3,Caspase-1,GSDMD,cGAS and STING pro-teins.Results:Compared with the control group,the A450 values(24 h,48 h)of H9c2 cells in the HR group were obviously reduced,the levels of LDH,IL-6,IL-18,IL-1β,PI staining positive rate,the expressions of cGAS,STING mRNA,the expressions of NLRP3,Caspase-1,GSDMD,cGAS,STING proteins were obviously increased(P<0.05);compared with the HR group,the A450 values(24 h,48 h)of H9c2 cells in the low and high dose irbesartan groups and the RU.521 group were obviously increased,the levels of LDH,IL-6,IL-18,IL-1β,PI staining positive rate,the expressions of cGAS,STING mRNA,the expressions of NLRP3,Caspase-1,GSDMD,cGAS,STING proteins were obviously reduced(P<0.05);RocA was able to partially reverse the protective effect of irbe-sartan on HR-induced myocardial cells(P<0.05);the detection indicators of H9c2 cells in the RU.521 group were at the same level as those in the high-dose irbesartan group(P>0.05).Conclusion:Irbesartan can inhibit HR-induced myocardial cell pyrop-tosis by inhibi-ting the cGAS-STING signaling pathway.关键词
厄贝沙坦/cGAS-STING信号通路/缺氧复氧/细胞焦亡Key words
Irbesartan/cGAS-STING signaling pathway/Hypoxia reoxygenation/Pyroptosis分类
医药卫生引用本文复制引用
刘玲玲,颜永进,王世亚,顾顺忠..厄贝沙坦调节cGAS-STING信号通路对缺氧复氧诱导的心肌细胞焦亡的影响[J].中国免疫学杂志,2025,41(11):2613-2618,6.基金项目
2023年度海安市高层次人才科研项目资助计划(202307) （202307）
2023年度南通市卫生健康委员会科研课题(MS2023101). （MS2023101）
