中国免疫学杂志2025，Vol.41Issue(11)：2695-2702,8.DOI:10.3969/j.issn.1000-484X.2025.11.023
血清let-7e与腹腔热灌注联合新辅助化疗治疗卵巢癌的疗效及其与Th17/Treg免疫相关因子水平的关系
Relationship between let-7e and curative effect of hyperthermic intraperitoneal perfusion combined with neoadjuvant chemotherapy on ovarian cancer and its relationship with Th17/Treg immune-related factor levels
摘要
Abstract
Objective:To explore relationship between let-7e and curative effect of hyperthermic intraperitoneal perfusion com-bined with neoadjuvant chemotherapy and Th17/Treg immune-related factor levels in patients with ovarian cancer.Methods:A total of 120 patients with ovarian cancer who received hyperthermic intraperitoneal perfusion combined with neoadjuvant chemotherapy Cang-zhou People's Hospital from September 2016 to September 2018 were selected as research objects,who were divided into effective group(n=96)and ineffective group(n=24)according to clinical efficacy.let-7e expression in patients with different clinical characteri-stics were compared.Hierarchical regression model was used to analyze relationship between let-7e expression and clinical characteri-stics.Data of patients with different curative effects and levels of Th17/Treg immune-related factors before and after treatment were compared.Logistic regression analysis and correction model were used to analyze relationship between let-7e expression and Th17/Treg immune-related factors.Correlation between let-7e expression and Th17/Treg immune-related factors and clinical efficacy was analyzed by subgroup analysis.Generalized linear mixed models(GLMMs)was used to analyze relationship between clinical efficacy and let-7e expression.Restricted cubic spline model was used to analyze association between let-7e expression and treatment response and dose-response relationship.Results:There were statistically significant differences in expression of let-7e in patients with different FIGO stages,tissue grades,number of pregnancies,number of abortions,operation time and blood loss(P<0.05).Hierarchical regression analysis showed that FIGO stage,number of abortions,operation time and blood loss had a significant negative effect on let-7e expres-sion(P<0.05),while tissue grade and number of pregnancies had a significant positive effect on let-7e expression(P<0.05).There were significant differences in age,FIGO stage,tissue grade,blood loss and let-7e expression among patients with different efficacy(P<0.05).After treatment,Th17,Treg,Th17/Treg,IL-17,IL-10 and TGF-β in effective group were significantly lower than before treatment(P<0.05),and Th17,Treg,IL-17,IL-10 and TGF-β in ineffective group were significantly lower than before treatment(P<0.05).After treatment,levels of Th17,Treg,Th17/Treg,IL-17,IL-10 and TGF-β in effective group were significantly lower than in-effective group(P<0.05).Correlation analysis showed that expression of let-7e was negatively correlated with Th17,Treg,Th17/Treg,IL-17,IL-10 and TGF-β(β<0,P<0.05).Relationship between let-7e expression and clinical efficacy was stable under different levels of Th17,Treg,Th17/Treg,IL-17,IL-10 and TGF-β(Ptrend<0.05),and there was no interaction between subgroups(Pinteraction>0.05).GLMMs analysis showed that with decrease of let-7e expression,risk of ineffective treatment increased significantly(P<0.05).Restricted cubic spline analysis showed that there was a nonlinear dose-response relationship between let-7e expression and treatment failure(P<0.05),with decrease of let-7e expression,risk of treatment failure increased significantly.Conclusion:With increase of let-7e expression,levels of Th17/Treg immune-related factors IL-17,IL-10 and TGF-β decrease,and hyperthermic intraperitoneal perfusion combined with neoadjuvant chemotherapy improves efficacy of ovarian cancer.关键词
let-7e/腹腔热灌注联合新辅助化疗/卵巢癌/疗效/Th17/TregKey words
let-7e/Hyperthermic intraperitoneal perfusion combined with neoadjuvant chemotherapy/Ovarian cancer/Cura-tive effect/Th17/Treg分类
临床医学引用本文复制引用
陈玲,王衍..血清let-7e与腹腔热灌注联合新辅助化疗治疗卵巢癌的疗效及其与Th17/Treg免疫相关因子水平的关系[J].中国免疫学杂志,2025,41(11):2695-2702,8.基金项目
沧州市重点研发计划自筹项目(192106015). （192106015）
