中国蔬菜Issue(11)：32-38,7.DOI:10.19928/j.cnki.1000-6346.2025.3033
基于成像流式细胞术评估斧翅沙芥种质资源基因组大小及居群间差异
Evaluation of Genome Size and Inter Population Differences of Pugionium dolabratum Germplasm Based on Imaging Flow Cytometry
摘要
Abstract
Pugionium dolabratum is a kind of rare and endangered Cruciferous germplasm,exhibiting multi-stress resistance such as drought and saline-alkali tolerance,with significant ecological and agricultural value.Imaging flow cytometry can distinguish nuclear signals from non-nucleus interference signals,resulting in higher accuracy.To investigate the genome sizes and their variation among different populations of P.dolabratum,this study employed imaging flow cytometry to analyze the nuclei from five P.dolabratum populations.The estimated genome sizes were 416-494 Mb,with a variation of 18.8%,indicating significant genetic differences among different populations.Four populations were surveyed using high-throughput sequencing,and the predicted genome sizes were 442-484 Mb.The results of the both methods were relatively similar(consistency 93%-97%,when heterozygosity＜2%),confirming the reliability of imaging flow cytometry.For the genome heterozygosity exceeds 2%,the difference between the two methods reached 16%,indicating that it is necessary to use imaging flow cytometry to evaluate genome size in plants with high heterozygosity.The results provide technical support and data basis for genome sequencing,germplasm protection on P.dolabratum.关键词
斧翅沙芥/成像流式细胞术/基因组大小/K-mer分析/濒危种质资源Key words
Pugionium dolabratum/imaging flow cytometry/genome size/K-mer analysis/endangered germplasm引用本文复制引用
李晨,张彤,张晓辉,王海平,宋江萍,阳文龙,贾会霞,张凤兰,张静,陈宜男,李苗苗..基于成像流式细胞术评估斧翅沙芥种质资源基因组大小及居群间差异[J].中国蔬菜,2025,(11):32-38,7.基金项目
国家重点研发计划项目(2021YFD1200201) （2021YFD1200201）
