中国蔬菜Issue(11)：142-149,8.DOI:10.19928/j.cnki.1000-6346.2025.5040
氮离子注入黄瓜的生物学效应
Biological Effects of Nitrogen Ion Implantation on Cucumber
摘要
Abstract
To investigate the effects of nitrogen ion implantation on cucumbers,this study utilized nitrogen ions with an energy of 30 keV to implant seeds of the cucumber inbred line ZOC at doses of 1.0×1015、2.0×1015、3.0×1015、4.0×1015、5.0×1015、6.0×1015 ions·cm-2.Biological indicators including seed vitality(germination rate,germination potential,germination index),seedling growth(plant height,root length),physiological activity(reactive oxygen species generation,antioxidant enzyme activity),and genetic material variation(RAPD variation degree)were measured.The results showed that as the implantation dose increased,seed vitality gradually declined.Based on seed germination and seedling growth performance,the optimal dose for nitrogen ion implantation in cucumbers was determined to be 3.5×1015 ions·cm-2.Seedling growth and antioxidant capacity initially increased and then decreased with rising implantation doses,peaking at 3.0×1015 to 4.0×1015 ions·cm-2.Due to cellular damage caused by ion implantation and changes in antioxidant capacity,reactive oxygen species generation exhibited a trend of increase-decrease-increase.The overall genetic variation rate was 33.33%,with the majority of variations attributed to gene fragment insertions and deletions.The experimental results provide reference data for studying nitrogen ion implantation-induced mutagenesis in cucumber breeding and lay a foundation for further research.关键词
黄瓜/氮离子注入/生物学效应Key words
cucumber/nitrogen ion implantation/biological effects引用本文复制引用
曹天光,范云鹏,秦垒,刘丽丽,鲍子涵,张航毓,耿金鹏..氮离子注入黄瓜的生物学效应[J].中国蔬菜,2025,(11):142-149,8.基金项目
河北省重点研发计划项目(21326312D,21326342D) （21326312D,21326342D）
