中国比较医学杂志2025，Vol.35Issue(10)：1-10,10.DOI:10.3969/j.issn.1671-7856.2025.10.001
清心运脾方治疗食物过敏相关特应性皮炎小鼠皮损改善及抗炎作用研究
Anti-inflammatory effects and amelioration of skin lesions by Qingxin Yunpi Formula in mice with food allergic atopic dermatitis
摘要
Abstract
Objective To investigate the therapeutic efficacy of Qingxin Yunpi Formula(QXYPF)in a mouse model of food allergy(FA)-associated atopic dermatitis(AD)induced by 2,4-dinitrochlorobenzene(DNCB)and ovalbumin(OVA).Methods Male BALB/c mice were randomly divided into six groups(n=10 per group):control,AD,model,QXYPF low-dose(QXYPF-L),QXYPF high-dose(QXYPF-H),and prednisone(PNS).Except for the control and AD groups,FA-AD was induced by epicutaneous application of DNCB on the dorsum combined with intraperitoneal OVA injection;the AD group received DNCB application only.Post-modeling assessments included:clinical evaluation of dorsal lesions,dermatitis severity scoring,quantification of scratching behavior,histopathological examination of skin sections to determine epidermal thickness and mast cell infiltration,enzyme-linked immunosorbent assay(ELISA)measurement of serum OVA-specific IgE(OVA-sIgE)levels,and of T-helper(Th)2 cytokines(IL-4,IL-5)and Th1 cytokine(IFN-γ),and immunohistochemical analysis of IL-4,IL-5,and IFN-γ in lesioned skin tissues.Results Compared with the control group,mice in the model groups exhibited more severe dorsal skin lesions,increased dermatitis scores and scratching frequencies(P＜0.05).The model group showed significantly thickened dorsal epidermis(P＜0.05),acanthosis,and noticeable inflammatory cell infiltration in the dermis.Serum levels of OVA-sIgE,IL-4,and IL-5 were elevated(P＜0.05),while IFN-γ levels were decreased in both AD and model groups(P＜0.05).Additionally,protein expression of IL-4 and IL-5 in dorsal lesional tissues was increased in the model group(P＜0.05),whereas IFN-γ protein levels were reduced in both AD and model groups(P＜0.05).In comparison with the model group,all treatment groups demonstrated improved dorsal skin lesions,significantly reduced dermatitis scores and scratching frequencies(P＜0.05),markedly alleviated epidermal thickening(P＜0.05),improved acanthosis,and reduced inflammatory infiltration in the dermis.Serum OVA-sIgE levels were decreased(P＜0.05),along with reduced serum IL-4 and IL-5 levels and increased IFN-γlevels(P＜0.05).Protein expression of IL-4 and IL-5 in lesional skin tissues was downregulated,while IFN-γ protein expression was upregulated(P＜0.05).Conclusions QXYF ameliorates skin lesions and pruritus in FA-AD mice,potentially by inhibiting Th2-related cytokines and promoting Th1-related cytokine expression.关键词
清心运脾方/特应性皮炎/食物过敏/Th1/Th2/细胞因子Key words
Qingxin Yunpi Formula/atopic dermatitis/food allergy/Th1/Th2/cytokines分类
中医学引用本文复制引用
尹一,翟盼盼..清心运脾方治疗食物过敏相关特应性皮炎小鼠皮损改善及抗炎作用研究[J].中国比较医学杂志,2025,35(10):1-10,10.基金项目
国家自然科学基金(82405482) （82405482）
河南中医药大学第一附属医院专业学位研究生精品教学案例项目(YJSJPAL-202213). （YJSJPAL-202213）
