中国比较医学杂志2025，Vol.35Issue(10)：20-27,8.DOI:10.3969/j.issn.1671-7856.2025.10.003
唾液酸干预对孤独症模型大鼠肠道功能的影响
Effects of sialic acid intervention on intestinal function in autism model rats
摘要
Abstract
Objective To explore the effects of sialic acid intervention during pregnancy and the lactational period on the intestinal function of autism model rats.Methods Thirty SPF-grade adult male and female Wistar rats were mated.The successfully pregnant rats were randomly assigned to a valproate-induced model(VPA)group,a high-dose sialic acid(SAH)group,a medium-dose sialic acid(SAM)group,a low-dose sialic acid(SAL)group,and a control(CON)group(n=6 per group)and were housed individually in single cages.On the 12.5th day of pregnancy(E12.5),rats in the VPA and SA intervention groups were given a single intraperitoneal injection of 600 mg/kg sodium valproate(VPA),while the pregnant rats in the CON group were given an equal amount of normal saline.The SA intervention period was from E12.5 to the 21st day after parturition.Feces of offspring rats in each group were collected.The diversity and structure of the gut microbiota were detected by 16S rRNA sequencing.The intestinal transit speed in each group was detected by intragastric administration of carmine.The levels of intestinal-related neurotransmitters(substance P,enkephalin,5-hydroxytryptamine,vasoactive intestinal peptide,and glutamate,gamma-aminobutyric acid)in the blood of rats in each group were detected by ELISA.Results High-dose SA intervention did not affect the diversity of the gut microbiota in the VPA-induced autism model rats,but it changed the structure of the gut microbiota and increased the abundance levels of Prevotella_NK3B31 group,Prevotella,Prevotella spp.,Alloprevotella,Lachnospira,Ruminococcus,and Bacteroides(P＜0.05).High-dose SA also promoted the intestinal transit speed,and increased the levels of vasoactive intestinal peptide,5-hydroxytryptamine,and gamma-aminobutyric acid in serum.Conclusions SA intervention during pregnancy and the lactational period affects the intestinal transit speed of VPA-induced autism model rats,changes the structure of the gut microbiome,and increases the levels of vasoactive intestinal peptide,5-hydroxytryptamine,and gamma-aminobutyric acid.关键词
唾液酸/孤独症谱系障碍/肠道菌群/大鼠/肠道功能Key words
sialic acid/autism spectrum disorder/gut microbiota/rats/intestinal function分类
临床医学引用本文复制引用
杨超,李洪杰,李刚,高悦,陈美琪,杨晓蕾..唾液酸干预对孤独症模型大鼠肠道功能的影响[J].中国比较医学杂志,2025,35(10):20-27,8.基金项目
国家自然科学基金(82103869) （82103869）
黑龙江省省属本科高校基本科研业务费项目(2024-KYYWF-0364). （2024-KYYWF-0364）
