中国兽医科学2025，Vol.55Issue(11)：1429-1436,8.DOI:10.16656/j.issn.1673-4696.2025.0193
柔嫩艾美耳球虫丝氨酸蛋白酶抑制剂1对宿主细胞凋亡的影响
Effect of Eimeria tenella serine protease inhibitor 1 on host cell apoptosis
摘要
Abstract
To explore the effect of Eimeria tenella serine protease inhibitor 1(EtSerpin 1)on host cell apoptosis,the EtSerpin1 protein was expressed using the Pichia pastoris system and added to the culture medium at an optimal concentration.Primary chicken embryo cecal epithelial cells infected with Eimeria tenella were then cultured.The effects of EtSerpin1 on parasite infection rate,apoptosis rate,Caspase-3 activity,and expression of apoptosis-related factors in host cells were assessed using HE staining,three-color fluorescence staining with Hoechst 33342 and Annexin V-FITC/PI,qRT-PCR,and Western-blot analysis.The results indicated that the parasite infection rate and Bcl-2 mRNA/protein levels of EtSerpinl treatment group were significantly increased(P＜0.01)at 4,24,and 96 h post-infe-ction,compared to the Eimeria tenella-only group,but apoptosis rate,Caspase-3 activity,and Bax mRNA/protein levels decreased significantly(P＜0.05)or highly significantly(P＜0.01).These find-ings suggested that EtSerpin1 can enhanced parasite infection rates in host cells and inhibited host cell apoptosis induced by Eimeria tenella.关键词
柔嫩艾美耳球虫/丝氨酸蛋白酶抑制剂1/细胞凋亡Key words
Eimeria tenella/serine protease inhibitor 1/cell apoptosis分类
农业科技引用本文复制引用
王吉霞,崔小珍,王明晓,王雨婷,徐梦博,赵睿,杨裕,关文超,白瑞,张黎..柔嫩艾美耳球虫丝氨酸蛋白酶抑制剂1对宿主细胞凋亡的影响[J].中国兽医科学,2025,55(11):1429-1436,8.基金项目
国家自然科学基金项目(31972647) （31972647）
山西省重点研发计划项目(2022ZDYF126) （2022ZDYF126）
山西农业大学科技创新提升工程项目(CXGC2023019) （CXGC2023019）
