中国兽医科学2025，Vol.55Issue(11)：1453-1461,9.DOI:10.16656/j.issn.1673-4696.2025.0205
PEDV N蛋白重组减毒沙门菌SC3(pYA3342-N)的构建及免疫效果评价
Construction of a recombinant attenuated Salmonella strain SC3(pYA3342-N)of PEDV N protein and evaluation of its immune effect
摘要
Abstract
To preliminarily evaluate the vaccine potential of the porcine epidemic diarrhea virus nucleocapsid(PEDV N)gene,this study utilized recombinant attenuated Salmonella to express the N protein and assessed immune responses in immunized mice.The N gene from the PEDV LJX strain was cloned into the pYA3342 vector via homologous recombination and electroporated into attenuated Salmonella SC3,generating the recombinant strain SC3(pYA3342-N).Stable genetic expression of PEDV N in SC3 was confirmed by RT-PCR and Western-blot.Following safety validation,7-week-old male BALB/c mice were immunized for immune evaluation.RT-qPCR analysis revealed significantly elevated IFN-γ levels in the SC3(pYA3342-N)and SC3 groups compared to the blank control group,indicating bacterial colonization in intestinal tissues and subsequent induction of cellular immunity.ELISA results demonstrated that SC3(pYA3342-N)-immunized mice exhibited markedly higher serum IgG and mucosal sIgA antibody levels than both the SC3 and control groups,with serum IgG titers showing a dose-dependent increase upon booster immunizations.These findings collectively indicate that the recombinant attenuated Salmonella SC3(pYA3342-N)effectively triggers robust cellular,humoral,and mucosal immune responses.This study provides preliminary evidence supporting PEDV N as a promising vaccine candidate and lays the foundation for developing oral live-vectored vaccines against PEDV.关键词
猪流行性腹泻病毒/N蛋白/重组减毒沙门菌/免疫效果评价Key words
porcine epidemic diarrhea virus/N protein/recombinant attenuated Salmonella/evaluation of immune effect分类
农业科技引用本文复制引用
宋艳雯,胡霞,杨柳,宋振辉..PEDV N蛋白重组减毒沙门菌SC3(pYA3342-N)的构建及免疫效果评价[J].中国兽医科学,2025,55(11):1453-1461,9.基金项目
中央高校基本业务专项(XDJK2020RC001) （XDJK2020RC001）
国家生猪技术创新中心项目(NCTIP-XD/B11) （NCTIP-XD/B11）
