中国兽医科学2025，Vol.55Issue(11)：1462-1469,8.DOI:10.16656/j.issn.1673-4696.2025.0196
蛋内沙门菌新型增菌方法的建立及其在Sm24/Rif12/Ssq活疫苗降低散养蛋鸡污染率中的应用
Establishment of a new method for increasing Salmonella in eggs and its application in the detection of Sm24/Rif12/Ssq live vaccine for reducing the contamination rate of free-range laying hens
摘要
Abstract
This study aims to develop a novel method for enhancing the detection of Salmonella in eggs and to evaluate its application in reducing the contamination rate of free-range laying hens vaccinated with the live vaccine Sm24/Rif12/Ssq.To improve the sensitivity of detection for low-level contamination of Salmonella and to resuscitate bacteria in a viable but non-culturable(VBNC)state,TSB pre-enrichment broth was supplemented with 35 μg/mL ferrous sulfate and 2 mmol/L sodium pyruvate.Over a six-month period(from August 2023 to January 2024),40 eggs were randomly sampled every 15 days(a total of 480 eggs)for Salmonella isolation and identification.Additionally,at each sampling time,10 environmental samples were collected using cotton swabs moistened with 0.1％peptone water from the chicken coop.The results demonstrated that the modified TSB(mTSB)significantly enhanced the detection rate of Salmonella in eggs and exhibited superior recovery of Salmonella in the VBNC state compared to standard TSB enrichment(P＜0.01).No Salmonella was detected in the eggs or eggshells of free-range hens vaccinated with the Sm24/Rif12/Ssq vaccine.This study confirms that mTSB is effective for isolating Salmonella with low contamination levels and VBNC status in eggs.Furthermore,the live Salmonella Enteritidis vaccine Sm24/Rif12/Ssq reduces the risk of Salmonella contamination in free-range local breed eggs,providing a reliable strategy for ensuring the safety of egg products in free-range laying hens.关键词
肠炎沙门菌/活疫苗/鸡蛋污染/散养/VBNCKey words
Salmonella Enteritidis/live vaccine/egg contamination/free-range farming/VBNC分类
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齐志颖,余祖华,陈松彪,张宁,耿云腾,贾艳艳,尚珂,廖成水,丁轲,郭荣显..蛋内沙门菌新型增菌方法的建立及其在Sm24/Rif12/Ssq活疫苗降低散养蛋鸡污染率中的应用[J].中国兽医科学,2025,55(11):1462-1469,8.基金项目
国家自然科学基金项目(32072771) （32072771）
河南科技大学博士科研启动基金项目(13480110) （13480110）
