中国兽医科学2025，Vol.55Issue(11)：1487-1493,7.DOI:10.16656/j.issn.1673-4696.2025.0210
赤羽病毒Gc蛋白的表达纯化及其单克隆抗体的制备
Expression and purification of Gc protein from Akabane virus and preparation of its monoclonal antibody
摘要
Abstract
To further investigate the infection mechanism of Akabane virus(AKAV)and the function of the Gc protein,the Gc protein of AKAV was expressed using a prokaryotic expression system.After purifica-tion,the Gc protein was used to immunize BALB/c mice.Following four immunizations,indirect ELISA screening,and cell fusion,a cell line capable of secreting monoclonal antibodies against the Gc protein was isolated,resulting in the production of monoclonal antibody 2D3,which was identified as IgG1/Kappa type.The results obtained from IFA,Western-blot,and indirect ELISA collectively showed that the monoclonal antibody 2D3 had a high specificity and affinity,and could specifically recognize the Gc protein of AKAV.The monoclonal antibody 2D3 against the Gc protein of AKAV was prepared,which provided a reliable tool for the establishment of AKAV detection methods as well as for the investigation of its pathogenic mechanisms.关键词
赤羽病毒/Gc蛋白/原核表达/单克隆抗体Key words
Akabane virus/Gc protein/prokaryotic expression/monoclonal antibody分类
畜牧业引用本文复制引用
盖文燕,郑海学,苏红,李国丽,徐斌,纪丽丽,齐茜,李汝春,何继军,朱伟..赤羽病毒Gc蛋白的表达纯化及其单克隆抗体的制备[J].中国兽医科学,2025,55(11):1487-1493,7.基金项目
"十四五"国家重点研发计划项目(2023YFD1801300) （2023YFD1801300）
国家绒毛用羊产业技术体系建设专项(CARS-39-13) （CARS-39-13）
甘肃省科技计划技术创新引导计划项目(25CXNJ009) （25CXNJ009）
山东省牛产业技术体系项目(SDAIT-09-07) （SDAIT-09-07）
山东畜牧兽医职业学院自然科学类重点课题(2022zrkxzd005) （2022zrkxzd005）
