中国兽医科学2025，Vol.55Issue(11)：1517-1524,8.DOI:10.16656/j.issn.1673-4696.2025.0199
一株新型鸭呼肠孤病毒的分离鉴定及致病性分析
Isolation,identification and pathogenicity analysis of a novel duck reo virus
摘要
Abstract
To investigate the prevalence of novel duck reovirus(NDRV),a viral strain was success-fully isolated using duck embryo propagation methods from the liver and spleen tissues of ducks suspected to be infected with NDRV in Weifang,Shandong Province.The isolate was confirmed as NDRV through RT-PCR,next-generation sequencing,homology comparison,and phylogenetic analysis of the S1 gene segment.The complete genome measured 23 419 bp in length,with the S1 gene encoding the P10,P18,and σ C proteins.Phy-logenetic analysis demonstrated that the isolate clustered within the NDRV reference strains available in the NCBI,sharing the highest nucleotide homology(98.1％—99.8％)with the NDRV J18 strain.The isolate was designated as NDRV-SDWF-01.To evaluate viral replication,the isolate was inoculated into leghorn male hepatoma(LMH)cells and serially passaged to the 5th generation(F5).Viral replication was confirmed by indirect immunofluorescence assay(IFA),with TCID50 determined as 1× 10-6.4/0.1 mL.Growth curves in BHK21,Vero,and LMH cells revealed superior adaptation to LMH cells,evidenced by specific green fluorescence.To further investigate the pathogenicity of the NDRV-SDWF-01 strain,3-day-old cherry valley ducklings were injected with 0.2 mL of F5 virus solution intramuscularly in the legs.The infected ducklings exhibited significant growth retardation,with their body weight consistently lower than that of the negative control group from day 3 post-inoculation onward.By day 7,one duckling succumbed to infection(10％mortality).Viral shedding was detectable as early as day 1 post-inoculation,peaked on day 5,and gradually declined thereafter.The results provide some reference for the analysis of NDRV genetic evolution and disease prevention and control.关键词
新型鸭呼肠孤病毒/分离鉴定/遗传进化分析/致病性Key words
novel duck reovirus/isolation and identification/genetic and evolutionary analysis/pathogenicity分类
畜牧业引用本文复制引用
刘国锰,冯晶晶,张武超,雷白时,袁万哲,陈福星,赵款..一株新型鸭呼肠孤病毒的分离鉴定及致病性分析[J].中国兽医科学,2025,55(11):1517-1524,8.基金项目
"预防兽医学与生物技术福建省高等学校重点实验室"开放基金课题项目(2021KF03) （2021KF03）
河北农业大学引进人才科研专项(YJ202020) （YJ202020）
