中国兽医科学2025，Vol.55Issue(11)：1551-1558,8.DOI:10.16656/j.issn.1673-4696.2025.0226
布劳恩脂蛋白对大肠杆菌感染的奶牛骨髓源巨噬细胞细胞因子分泌作用机制研究
Effect of BLP on cytokine secretion of bovine bone marrow-derived macrophages induced by E.coli infection
摘要
Abstract
To investigate the mechanism of action of Braun lipoprotein(BLP)in Escherichia coli-induced bovine endometritis,this study stimulated macrophages with LPS,BLP,and LPS combined with BLP;and infected bovine bone marrow-derived macrophages(bBMMs)with E.coli JE5505,E.coli DH5α,and E.coli JE5505 combined with BLP stimulation.ELISA,quantitative real-time PCR,and Western-blot techniques were employed to analyze cytokine secretion,the activation of inflammatory and anti-inflammatory signaling pathways,and the effect of TLR2 receptor activation on cytokine production.Compared with E.coli JE5505 infection group,bBMMs infected with E.coli DH5α group or infected with E.coli JE5505 combined with BLP stimulation group exhibited significantly decreased levels of pro-inflammatory cytokines TNF-α and IL-1β,while the secretion of the anti-inflammatory cytokine IL-10 was significantly elevated.Western-blot analysis demonstrated that BLP modulated the activation of the NF-κB/MAPK pathway as well as the expression of the anti-inflammatory signaling pathway STAT3 in bBMMs.These findings suggest that during E.coli infection,BLP is recognized by the TLR2 receptor and may attenuate the production of pro-in-flammatory cytokines and chemokines in bBMMs through the activation of the MAPK and NF-κB signaling pathways.Additionally,BLP may enhance the production of the anti-inflammatory cytokine IL-10 via the activation of the STAT3 signaling pathway,thereby mitigating the inflammatory response induced by E.coli infection.This indicates that BLP has potential therapeutic effects in the regulation of infection-induced inflammation.关键词
子宫内膜炎/大肠杆菌/布劳恩脂蛋白/脂多糖/奶牛骨髓源巨噬细胞Key words
endometritis/Escherichia coli/Braun lipoprotein/lipopolysaccharide/bovine bone marrow-derived macrophages分类
畜牧业引用本文复制引用
刘谕泽,刘博,毛伟,张双翼,巩志国,于琢雅,任佩佩,赵佳敏,王奕心,代佳山..布劳恩脂蛋白对大肠杆菌感染的奶牛骨髓源巨噬细胞细胞因子分泌作用机制研究[J].中国兽医科学,2025,55(11):1551-1558,8.基金项目
国家自然科学基金项目(32160854) （32160854）
