中国兽医杂志2025，Vol.61Issue(11)：49-55,7.DOI:10.20157/j.cnki.zgsyzz.2025.11.007
疫苗应用对规模化猪场猪圆环病毒2型感染压力和血清流行病学的影响
Effect of Vaccine Application on Porcine Circovirus Type 2 Infection Pressure and Seroepidemiology in Large-scale Pig Farms
摘要
Abstract
To evaluate the changes in infection pressure and seroepidemiology of porcine circovirus type 2(PCV2)before and after vaccination in large-scale pig farms,a total of 1 818 serum samples were collected from 48 farms across 4 regions in China.Indirect enzyme linked immunosorbent assay(ELISA)and real-time fluorescent quantitative polymerase chain reaction(qPCR)were employed to detect PCV2 antibody levels and infection status before vaccination,as well as after 5 and 10 years of vaccine application.The results indicated that(1)the average positive rate and S/P ratio of PCV2 capsid(Cap)antibodies in sows remained above 98.00%and 1.50,respectively,with no significant difference over time(P>0.05).However,after 10 years of vaccination,the average positive rate of PCV2 replicase(Rep)antibodies in sows significantly decreased from 97.77%to 76.50%,and the average S/P ratio declined from 0.71 to 0.53(P<0.05).(2)In fattening pigs,the average PCV2 Cap antibody positive rate showed no significant difference compared to the 5-year mark(P>0.05),but the S/P ratio decreased significantly(P<0.05).The PCV2 Rep antibody positive rate declined markedly from 81.96%to 30.92%,and the S/P ratio dropped from 0.76 to 0.28(P<0.01).(3)The PCV2 Rep antibody positivity and S/P value increased significantly after 22 weeks of age(P<0.05 and P<0.01,respectively),aligning with the PCV2 detection peak at 20-28 weeks.(4)Additionally,the PCV2 positivity rate in fattening pigs significantly decreased from 14.58%to 2.08%after 10 years of vaccination(P<0.05).These findings demonstrate that long-term vaccination can effectively reduce PCV2 infection pressure,and that Rep antibody dynamics may serve as a valuable indicator for evaluating the purification effect,providing both theoretical support and practical data for PCV2 prevention and control strategies.关键词
猪圆环病毒2型(PCV2)/疫苗免疫/流行病学调查/间接酶联免疫吸附试验Key words
porcine circovirus type 2(PCV2)/vaccine immunization/epidemiological investigation/indirect enzyme-linked immunosorbent assay分类
畜牧业引用本文复制引用
梁彤彤,罗烨,林美婷,张珊玲,冯泽中,郑金,卿任科,余兴龙..疫苗应用对规模化猪场猪圆环病毒2型感染压力和血清流行病学的影响[J].中国兽医杂志,2025,61(11):49-55,7.基金项目
湖南省重点领域研发计划项目(2022NK2049) （2022NK2049）
