中国兽医杂志2025，Vol.61Issue(11)：112-121,10.DOI:10.20157/j.cnki.zgsyzz.2025.11.015
皂角刺总皂苷的提取工艺、化学成分和体外抗炎活性研究
Study on the Extraction Process,Chemical Composition and in vitro Anti-inflammatory Activity of Total Saponins from Gleditsia sinensis Lam
摘要
Abstract
To explore the extraction process,chemical composition,and in vitro anti-inflammatory activity of total saponins from Gleditsia sinensis Lam.(GTS),and to provide a reference for the development of novel traditional Chinese veterinary additives,this study employed single-factor experiments and response surface methodology to optimize the extraction process of GTS.The chemical components of GTS were preliminarily identified using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS).Furthermore,the effects of GTS on cell viability,nitric oxide(NO)production,and phagocytic activity were evaluated using a lipopolysaccharide(LPS)-induced inflammation model in RAW 264.7 mouse macrophages.The optimal extraction conditions were determined as follows:ethanol concentration 44%,liquid-to-material ratio 50∶1(mL/g),extraction time 20 minutes,extraction temperature 40℃,and two extraction cycles,resulting in an average yield of(1.44±0.01)%.The major constituents of GTS were preliminarily identified as 13 saponins and 3 flavonoids.GTS showed no cytotoxicity to RAW 264.7 cells and significantly inhibited LPS-induced increases in NO production and phagocytic activity(P<0.01),with no significant difference compared to control drug dexamethasone(DEX)(P>0.05).These findings suggest that GTS possesses notable anti-inflammatory potential and may serve as a valuable candidate for the development of new Chinese veterinary medicine additives.关键词
皂角刺/总皂苷/提取工艺/化学成分/抗炎作用Key words
Gleditsia sinensis Lam./total saponins/extraction process/chemical composition/anti-inflammatory activity分类
畜牧业引用本文复制引用
朱彤,岳少阳,赵含羽,王誉瑾,吴东东,谢亚平,刘明春,田春莲..皂角刺总皂苷的提取工艺、化学成分和体外抗炎活性研究[J].中国兽医杂志,2025,61(11):112-121,10.基金项目
沈阳农业大学优秀本科毕业论文培育计划项目(2021032) （2021032）
辽宁省科学技术计划项目(2022-MS-257) （2022-MS-257）
沈阳农业大学中青年研究生导师培养资助项目(2022-ZZDS-14) （2022-ZZDS-14）
