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辣椒BAM家族基因鉴定及表达模式分析

李宁 尹延旭 高升华 王畅 葛长军 谢勇 王飞 姚明华

中国瓜菜2025,Vol.38Issue(11):13-25,13.
中国瓜菜2025,Vol.38Issue(11):13-25,13.DOI:10.16861/j.cnki.zggc.2025.0347

辣椒BAM家族基因鉴定及表达模式分析

Genome-wide identification and expression pattern analysis of BAM fam-ily gene in Capsicum annuum

李宁 1尹延旭 1高升华 1王畅 1葛长军 2谢勇 3王飞 1姚明华1

作者信息

  • 1. 蔬菜种质创新与遗传改良湖北省重点实验室·湖北省农业科学院经济作物研究所 武汉 430064
  • 2. 黄冈市农业科学院 湖北黄冈 438000
  • 3. 武汉楚为种业科技有限公司 武汉 430070
  • 折叠

摘要

Abstract

β-amylase(BAM)family gene is widely present in various plants and plays a significant role in plant growth and responses to abiotic stress.To further explore the information of the BAM family members in pepper and analyze their expression patterns and gene functions,bioinformatics methods were employed to conduct genome-wide identifica-tion,gene structure,phylogenetic analysis,and expression pattern analysis of the CaBAM gene family in pepper.The re-sults indicated that there were 10 CaBAM genes in pepper,all of which were hydrophilic proteins and distributed in the nu-cleus,cytoplasm,mitochondria,chloroplasts,and plasma membrane.Phylogenetic analysis divided the CaBAM gene fami-ly into 6 groups,and the conservation of catalytic residues Glu-186 and Glu-380 was higher than that of the flexible loop and inner loop.The promoter regions of CaBAM genes contained multiple cis-acting elements related to stress and devel-opment.Transcriptome and qRT-PCR analysis showed that CaBAM genes had differential expression in different tissues and under abiotic stress,and CaBAM4,CaBAM5,and CaBAM6 might play roles in the growth and development of pepper and responses to abiotic stress.The research results provide a reference for further in-depth studies on the function of the CaBAM gene family in pepper.

关键词

辣椒/BAM基因家族/基因表达/生物信息学

Key words

Pepper/BAM gene family/Gene expression/Bioinformatics

分类

农业科技

引用本文复制引用

李宁,尹延旭,高升华,王畅,葛长军,谢勇,王飞,姚明华..辣椒BAM家族基因鉴定及表达模式分析[J].中国瓜菜,2025,38(11):13-25,13.

基金项目

湖北省现代农业产业技术体系专项资金资助(2024HBSTX4-06) (2024HBSTX4-06)

武汉市特派员产学研专项(2023110201030679) (2023110201030679)

湖北省农业科技创新中心创新团队项目(2025-620-000-001-01,2025-620-000-001-007) (2025-620-000-001-01,2025-620-000-001-007)

中国瓜菜

OA北大核心

1673-2871

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