中国瓜菜2025，Vol.38Issue(11)：26-35,10.DOI:10.16861/j.cnki.zggc.2025.0287
马铃薯StWRKY15基因克隆与表达特征分析
Cloning and expression pattern analysis of StWRKY15 gene in potato
摘要
Abstract
WRKY transcription factors play a crucial role in the signal transduction pathways of plant disease resistance.Potato was selected as the model organism to investigate the properties and functions of StWRKY15.RNA-seq analysis identified one transcription factor,StWRKY15,as being significantly induced by potato early blight.StWRKY15 was suc-cessfully cloned using PCR techniques.The full-length sequence spans 1002 bp and encodes 333 amino acids.Subcellular localisation was confirmed to be within the nucleus.StWRKY15 belongs to the unstable hydrophilic protein family.It has a molecular mass of approximately 33.6 kDa and a theoretical pI of around 9.45.It exhibits the highest homology with CaWRKY15.Both proteins are members of the WRKY family.Analysis of StWRKY15 expression levels in various potato tissues using qRT-PCR revealed the highest expression in stems.The expression levels of StWRKY15 were measured at various time points after inoculation with Alternaria solani.The results indicated a significant increase in StWRKY15 ex-pression at 24 hours post-inoculation,with peak expression observed at 48 hours.This demonstrates that StWRKY15 is strongly induced by potato early blight.Transient overexpression established that StWRKY15 is associated with enhanced resistance to Alternaria solani in tobacco.These results lay the groundwork for further research into the role of St-WRKY15 in conferring resistance to early blight in potato.关键词
马铃薯/StWRKY15/生物信息学/亚细胞定位/瞬时过表达Key words
Potato/StWRKY15/Bioinformatics/Subcellular localization/Transient expression分类
农业科技引用本文复制引用
史梦祥,汪政,陈豪,丁丽丽,杨志辉,李倩..马铃薯StWRKY15基因克隆与表达特征分析[J].中国瓜菜,2025,38(11):26-35,10.基金项目
国家重点研发计划项目(2024YFD1401204) （2024YFD1401204）
国家现代农业产业技术体系建设专项资金(CARS-09-P18) （CARS-09-P18）
河北省薯类产业技术体系创新团队专项基金(HBCT2023060205) （HBCT2023060205）
河北农业大学引进人才科研专项(YJ2020041) （YJ2020041）
