中国瓜菜2025，Vol.38Issue(11)：65-76,12.DOI:10.16861/j.cnki.zggc.2025.0171
应用响应面法优化马铃薯疮痂病生防链霉菌PBSH9发酵培养基
Optimizing the fermentation medium of biocontrol Streptomyces PBSH9 against potato common scab using response surface methodology
摘要
Abstract
Potato common scab is a significant disease that restricts the development of the potato industry.This study screened the biocontrol strain PBSH9,and its metabolites showed significant inhibitory effects on the pathogen,achieving a field control efficacy of 73.97％.To identify the active compounds,the secondary metabolites of strain PBSH9 were iso-lated and purified,successfully yielding the active substance.To enhance the yield of this active substance,response sur-face methodology(RSM)was employed to optimize its culture medium.Based on single-factor experiments and Plack-ett-Burman tests,combined with the steepest ascent method and Box-Behnken design response surface methodology to fit the nonlinear equation between significant factors and the yield of active compound,the optimal fermentation medium for-mula was obtained.The optimized formula was 4.50 g·L-1 glucose,50.00 g·L-1 soluble starch,15.00 g·L-1 corn flour,21.08 g·L-1 soybean meal,2.00 g·L-1 peptone,0.50 g·L-1(NH4)2SO4,0.60 g·L-1 MgSO4,and 0.04 g·L-1 KH2PO4.The mod-el-predicted yield of active compounds was 199.92 μg·mL1,while the actual value was 184.35 μg·mL-1,which was 5.83 times higher than the yield of compounds in the basic culture medium.This study provides crucial technical sup-port for the development of efficient biopesticide products against potato common scab.关键词
马铃薯疮痂病/生防链霉菌/响应面法/活性物质/培养基Key words
Potato common scab/Biocontrol Streptomyces/Response surface methodology/Bioactive metabolites/Medium分类
农业科技引用本文复制引用
张敏,万中义,方伟,张亚妮,刘芳,柯少勇,张志成,董永义,张笑宇..应用响应面法优化马铃薯疮痂病生防链霉菌PBSH9发酵培养基[J].中国瓜菜,2025,38(11):65-76,12.基金项目
国家自然科学基金(32260700) （32260700）
内蒙古自治区科技计划项目(2022YFYZ0008) （2022YFYZ0008）
内蒙古自治区特色品种育种攻关联合项目(YZ2023006) （YZ2023006）
