中国瓜菜2025，Vol.38Issue(11)：77-84,8.DOI:10.16861/j.cnki.zggc.2025.0003
表油菜素内酯对干旱胁迫条件下黄瓜幼苗的影响
Effects of epibrassinolide on cucumber seedlings under drought stress
摘要
Abstract
Drought is one of the major abiotic stresses affecting plant growth and development.This study aims to investi-gate the effects of exogenous 2,4-epibrassinolide(EBR)on the growth,physiological characteristics,antioxidant defense system,and key genes in the MAPK cascade response of cucumber seedlings under drought stress.The cucumber variety Jinyou 186 was used as the experimental material,and four treatments were set up:Distilled water spraying(CK),50 mmol·L-1 PEG(PEG),50 mmol·L-1 PEG+0.02 mmol·L-1 EBR spraying(PEG+EBR),and 50 mmol·L-1 PEG+24 mmol·L-1 brassinosteroid biosynthesis inhibitor spraying(PEG+BRZ).The results showed that compared with the PEG treatment,the PEG+EBR treatment significantly increased the plant height,fresh mass of the aboveground part,fresh mass of the underground part,total root length,total root surface area,and total chlorophyll content of cucumber seedlings after 48 h of treatment.At 24 h of treatment,compared with the PEG treatment,the PEG+EBR treatment signifi-cantly increased the activity of catalase by 27.86％and decreased the activity of peroxidase by 41.58％,while the content of soluble sugar and proline were significantly increased by 51.47％and 30.69％,respectively,and the relative conductivity was significantly decreased by 22.22％.In addition,EBR induced the up-regulation of the expression of CsMAPK6 and CsMAPK9 genes in the MAPK cascade signaling pathway,thereby enhancing the drought tolerance of cucumber.In sum-mary,exogenous EBR application can effectively alleviate the damage of drought stress to cucumber seedlings by enhanc-ing antioxidant capacity and activating drought-related signaling pathways,and promote the growth and root system development of cucumber seedlings.The results of this study provide a theoretical basis for the use of exogenous EBR to improve the drought tolerance of cucumber seedlings.关键词
黄瓜/干旱胁迫/表油菜素内酯/抗氧化/MAPKKey words
Cucumber/Drought Stress/Epibrassinolide/Antioxidant/MAPK分类
农业科技引用本文复制引用
张国恒,李荣峰,田玲..表油菜素内酯对干旱胁迫条件下黄瓜幼苗的影响[J].中国瓜菜,2025,38(11):77-84,8.基金项目
山西省重点研发计划(农业领域)项目(201803D221004-3) （农业领域）
