中国药房2025，Vol.36Issue(22)：2772-2777,6.DOI:10.6039/j.issn.1001-0408.2025.22.04
基于血清药物化学与网络药理学的鸡骨草和毛鸡骨草抗肝炎作用机制研究
Study on the anti-hepatitis mechanism of Abrus pulchellus subsp.cantoniensis(Hance)Verdc.and Abrus pulchellus subsp.mollis(Hance)Verdc.based on serum pharmacochemistry and network pharmacology
摘要
Abstract
OBJECTIVE To compare the anti-hepatitis mechanisms of Abrus pulchellus subsp.cantoniensis(Hance)Verdc.(AC)and Abrus pulchellus subsp.mollis(Hance)Verdc.(AM).METHODS SD rats were randomly divided into blank group,AC-treated group,and AM-treated group,with each group consisting of 10 rats.The rats'orbital venous blood was collected at 5,15,30 minutes,and 1,1.5,2,4,6,8,12 hours after gavage administration of 24 g/kg of the corresponding drug(calculated by crude drug)or water,respectively.Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry technology was utilized to identify the prototype components present in the serum.The network pharmacology method was adopted to predict the anti-hepatitis active components,key targets,and signaling pathways of AC and AM.Additionally,molecular docking technology was utilized to verify the binding activity of the core active components with key targets.RESULTS A total of 35 prototype components migrating to the blood of AC and AM were identified in the serum of administered rats,among which 24 were common components.The active components in AC,such as acetylanguidine,physcion,soyasaponin A3 and soyasaponin Ⅰ,as well as those in AM,including vicenin 3,acetylanguidine,soyasaponin Ⅰ and schaftoside,all acted on key targets such as steroid receptor coactivator,phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha,epidermal growth factor receptor(EGFR),and protein kinase B1(Akt1).These components modulated pathways in cancer,EGFR tyrosine kinase inhibitor resistance,and the phosphoinositide 3-kinase(PI3K)-Akt pathway,thereby exerting anti-hepatitis effects.Furthermore,the binding energies between these active components and their key targets were all less than-5 kJ/mol.CONCLUSIONS There are differences in the active components of AC and AM against hepatitis,but their mechanisms of action are similar.Both may exert their anti-hepatitis effects through pathways in cancer,EGFR tyrosine kinase inhibitor resistance,and the PI3K-Akt pathway.关键词
鸡骨草/毛鸡骨草/抗肝炎/血清药物化学/网络药理学/分子对接/作用机制Key words
Abrus pulchellus subsp.cantoniensis(Hance)Verdc./Abrus pulchellus subsp.mollis(Hance)Verdc./anti-hepatitis/serum pharmacochemistry/network pharmacology/molecular docking/mechanism of action分类
中医学引用本文复制引用
周巧雯,王雪,黄明娟,黎理,陈文雅,王峥涛,张紫佳,樊兰兰..基于血清药物化学与网络药理学的鸡骨草和毛鸡骨草抗肝炎作用机制研究[J].中国药房,2025,36(22):2772-2777,6.基金项目
国家自然科学基金项目(No.82360739) （No.82360739）
广西科技计划项目(No.桂科AA23023035) （No.桂科AA23023035）
广西壮族自治区高校黄大年式教师团队"中药学传承创新教师团队"项目(No.桂教教师[2023]31号) （No.桂教教师[2023]31号）
广西中医药大学-柳药集团青年科技创新能力提升计划专项(No.YXY0000303) （No.YXY0000303）
上海市复方中药重点实验室(上海中医药大学)开放基金项目(No.2023OPCCMSHUTCM-06) （上海中医药大学）
