中国药房2025，Vol.36Issue(22)：2796-2801,6.DOI:10.6039/j.issn.1001-0408.2025.22.08
红景天苷通过VIP-cAMP通路对大鼠腮腺放射性损伤的改善作用及机制
Improvement effect and mechanism of salidroside on radiation-induced parotid gland injury in rats by VIP-cAMP pathway
摘要
Abstract
OBJECTIVE To explore the improvement effect and mechanism of salidroside on radiation-induced parotid gland injury in rats.METHODS Rats were randomly assigned into normal group,radiation group,salidroside low-dose(salidroside-L,50 mg/kg)group,salidroside high-dose(salidroside-H,100 mg/kg)group,and salidroside-H+inhibitor(100 mg/kg salidroside+0.1 µmol/kg H-89)group,with 10 rats in each group.Except for the normal group,rats in the other groups were subjected to radiation exposure to establish a model of radiation-induced parotid gland injury.Rats in each group were intraperitoneally injected with the corresponding drug or normal saline,once a day,for 40 consecutive days.After the last administration,the levels of reactive oxygen species(ROS),cyclic adenosine monophosphate(cAMP),superoxide dismutase(SOD),and amylase in serum were detected;the pathological changes of parotid gland tissue were observed;the apoptosis rate of parotid gland tissue cells,the expression levels of B-cell lymphoma-2(Bcl-2)and its associated X protein(Bax),mRNA expression levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α),the protein expression levels of type Ⅲ collagen(Col Ⅲ),vasoactive intestinal peptide(VIP),and the phosphorylation level of protein kinase A(PKA)in parotid gland tissue were determined.RESULTS Compared with normal group,the levels of ROS,amylase,apoptosis rate,Bax expression level,mRNA expression levels of IL-6 and TNF-α,and protein expression level of Col Ⅲ in the radiation group were significantly increased,while the levels of cAMP,SOD,Bcl-2 expression level,VIP protein expression level and PKA phosphorylation level were significantly decreased(P＜0.05).Compared with radiation group,the above indicators in the salidroside-L group and salidroside-H group were significantly improved(P＜0.05),and the improvement in the salidroside-H group was more significant(P＜0.05);inhibitor H-89 significantly reversed the changes in the above indicators of the salidroside-H group(P＜0.05).CONCLUSIONS Salidroside can effectively alleviate radiation-induced parotid gland injury in rats,and its mechanism may be related to the activation of the VIP-cAMP pathway.关键词
红景天苷/放射性损伤/腮腺/VIP-cAMP通路Key words
salidroside/radiation injury/parotid gland/VIP-cAMP pathway分类
医药卫生引用本文复制引用
张春英,阴广维,陈红,尤鸣达,刘金凤,张亚昆,胡耀杰..红景天苷通过VIP-cAMP通路对大鼠腮腺放射性损伤的改善作用及机制[J].中国药房,2025,36(22):2796-2801,6.基金项目
河北省医学科学研究课题(No.20240944) （No.20240944）
