中国药房2025，Vol.36Issue(22)：2802-2808,7.DOI:10.6039/j.issn.1001-0408.2025.22.09
芦荟苷通过调控巨噬细胞极化改善动脉粥样硬化的机制研究
Mechanism of aloin ameliorating atherosclerosis through regulating macrophage polarization
摘要
Abstract
OBJECTIVE To investigate the mechanism by which aloin(ALO)ameliorates atherosclerosis(AS).METHODS Eight C57BL/6J mice were assigned to the control group(CON group)and fed a standard diet;thirty-two apolipoprotein E-knockout(APOE-/-)mice were randomly divided into model group(MOD group),ALO low-dose and high-dose groups[ALO-L group,ALO-H group,20,40 mg/(kg·d)],and atorvastatin positive control group[ATO group,4 mg/(kg·d)],with 8 mice in each group,establishing the AS model through feeding with a high-fat diet.The mice were administered the drug via gavage or given an equal volume of deionized water for 8 consecutive weeks.The lipid levels in the serum of mice were measured,and the pathological structural changes in their aortas were observed.The expressions of macrophage polarization markers(CD86+,CD206+)in the aorta were determined,along with the mRNA expressions of inducible nitric oxide synthase(iNOS),tumor necrosis factor-α(TNF-α),arginase-1(Arg-1),and interleukin-10(IL-10),as well as the protein expressions of iNOS and Arg-1,and the phosphorylation levels of nuclear factor κB p65(NF-κB p65)and signal transduction and activator of transcription 3(STAT3)proteins.Additionally,a macrophage polarization model was established using lipopolysaccharide(LPS)-induced RAW264.7 cells,and the effect of ALO(400 μmol/L)on the cellular polarization phenotype was investigated.RESULTS Compared with the MOD group,administration groups all showed significant improvement in dyslipidemia(except for high-density lipoprotein cholesterol in the serum of ALO-L group)(P＜0.05);aortic intimal structure improved significantly,plaque area was reduced significantly(P＜0.01);the CD86+relative fluorescence intensity in the aorta decreased significantly,the CD206+relative fluorescence intensity increased significantly(P＜0.01),while the expressions of iNOS and TNF-α mRNA were down-regulated significantly(P＜0.05);mRNA expressions of Arg-1 and IL-10,and protein expression of Arg-1 were increased significantly in ALO-H group and ATO group(P＜0.05);the protein expressions of iNOS,and the phosphorylation levels of NF-κB p65 and STAT3 protein were decreased significantly(P＜0.05).In vitro experiments further confirmed that ALO significantly reduced the proportion of LPS-induced M1-type macrophages but increased the proportion of M2-type macrophages(P＜0.01).CONCLUSIONS ALO inhibits M1-type macrophage polarization and promotes M2-type polarization,ameliorates dyslipidemia and reduces arterial plaque formation in AS model mice,improve the structure of the aortic intima potentially through suppression of the NF-κB/STAT3 signaling pathway.关键词
芦荟苷/动脉粥样硬化/巨噬细胞极化/NF-κB/STAT3信号通路Key words
aloin/atherosclerosis/macrophage polarization/NF-κB/STAT3 signaling pathway分类
医药卫生引用本文复制引用
田野,陈线茹,梅向辉,李百超,杜文涛..芦荟苷通过调控巨噬细胞极化改善动脉粥样硬化的机制研究[J].中国药房,2025,36(22):2802-2808,7.基金项目
邯郸市科学技术研究与发展计划项目(No.19422083011-11) （No.19422083011-11）
