中国医学装备2025，Vol.22Issue(11)：56-60,5.DOI:10.3969/j.issn.1672-8270.2025.11.008
基于基质辅助激光解吸电离飞行时间质谱平台的耐碳青霉烯类肠杆菌耐药快速检测方法建立与应用
Establishment and application of rapid detection method based on MALDI-TOF MS platform for CRE drug resistance
摘要
Abstract
Objective:To establish a rapid detection method based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS)for drug resistance of Carbapenem-resistant Enterobacteriaceae(CRE),so as to provide basis for enhancing clinically diagnostic effect.Methods:A total of 120 suspected CRE strains that were isolated from the clinical laboratory of Jiading District Central Hospital Affiliated to Shanghai University of Medicine&Health Sciences during October 2023 and September 2024 were collected,and the experimental detection of 44 strains was finally completed.The 44 suspected CRE bacterial strains were all from hospitalized patients.A rapid detection protocol based on the MALDI-TOF MS platform was initially established and improved,and experimental detection was carried out using the fully automatic rapid microbial mass spectrometry detection system(VITEK MS).The results of identification review and antimicrobial susceptibility test(AST)for VitEK-2 Compact strain was used as the reference standard.The screening results and detection efficacy of two kinds of detection methods were compared.Results:In the 44 suspected CRE strains,31 CRE strains were screened out by MALDI-TOF MS detection,and 31 CRE stains were screened out by VITEK-2 Compact recheck detection.In them,one strain of Enterobacter cloacae was confirmed as negativity by the MALDI-TOF MS test,but it was confirmed as CRE by the VITEK-2 Compact test.One strain of Klebsiella pneumoniae was confirmed as positivity by the MALDI-TOF MS test,but it was confirmed as mediator for carbapenem drugs by the VITEK-2 Compact test.The tested results of VITEK-2 Compact was used as standard,the results of four-grid table(2×2 contingency table)statistical method indicated that the sensitivity,specificity,accuracy rate and Youden index(R)of MALDI-TOF MS were respectively 96.7%,92.3%,100%and 89.1%.Conclusion:The rapid detection method based on the MALDI-TOF MS platform for CRE drug resistance has simple operation process and high accuracy rate,which can provide a basis for the application of targeted antibacterial drugs at early stage.关键词
耐碳青霉烯类肠杆菌(CRE)/基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)/全自动快速微生物质谱检测系统(VITEK MS)/药敏试验(AST)Key words
Carbapenem-resistant Enterobacteriaceae(CRE)/Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)/Full automatic rapid microbial mass spectrometry detection system/Antimicrobial susceptibility test(AST)分类
医药卫生引用本文复制引用
刘业琼,唐文鑫,张洪霖,严峰,徐炜新..基于基质辅助激光解吸电离飞行时间质谱平台的耐碳青霉烯类肠杆菌耐药快速检测方法建立与应用[J].中国医学装备,2025,22(11):56-60,5.基金项目
上海市嘉定区自然科学基金课题(JDKW-2022-0014) Nature Science Research Projects in Shanghai Jiading District(JDKW-2022-014) （JDKW-2022-0014）
