中兽医医药杂志2025，Vol.44Issue(6)：46-51,6.DOI:10.13823/j.cnki.jtcvm.2025.072
单宁酸对金黄色葡萄球菌的体内外抑菌作用研究
In vitro and in vivo antibacterial effect of tannic acid against Staphylococcus aureus
摘要
Abstract
To evaluate the in vitro and in vivo antibacterial effects of tannic acid against Staphylococcus aureus,the broth microdilution method was used to determine the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of tannic acid against methicillin-resistant Staphylococcus aureus(MRSA)USA300.Subsequently,crystal violet staining,scanning electron microscopy(SEM),and laser confocal microscopy were used to analyze the effect of tannic acid at 1/2 MIC on the formation of MRSA USA300 biofilms.Finally,a murine mastitis model(MRSA USA300 infection)was established,and hematoxylin-eosin(H-E)staining,ELISA kits,and real-time quantitative PCR were used to evaluate the therapeutic effect of tannic acid on mouse mastitis.The results showed that the MIC was 0.625 mg/mL and the MBC was 1.250 mg/mL.Tannic acid at 1/2 MIC(0.312 5 mg/mL)could significantly inhibit the quantity of MRSA USA300 biofilm(P<0.01)and interfere in the formation of its three-dimensional structure.Tannic acid could effectively alleviate congestion and swelling in the mammary tissue of infected mice,reduce the damage to alveolar epithelial cells,and decrease inflammatory cell infiltration.It could also reduce the levels of the inflammatory cytokines TNF-α and IL-6 in mammary tissue(P<0.05)and inhibit the gene expression of TNF-α and IL-6(P<0.05).These findings indicate that tannic acid exhibits strong antibacterial activity against Staphylococcus aureus both in vitro and in vivo.This provides a scientific basis for further elucidating the antibacterial mechanism of tannic acid and for developing drugs to treat MRSA infection.关键词
单宁酸/金黄色葡萄球菌/生物被膜/小鼠乳腺炎Key words
tannic acid/Staphylococcus aureus/biofilm/murine mastitis分类
畜牧业引用本文复制引用
向佳佳,李发敏,杨奕樱,徐昌君,李军,周永辉,王爽..单宁酸对金黄色葡萄球菌的体内外抑菌作用研究[J].中兽医医药杂志,2025,44(6):46-51,6.基金项目
贵州中医药大学大学生创新创业训练计划项目[贵中医大创合字2024(178号)] （178号）
贵州省科技计划项目(黔科合基础-ZK[2024]一般349) （黔科合基础-ZK[2024]一般349）
