中兽医医药杂志2025，Vol.44Issue(6)：58-62,5.DOI:10.13823/j.cnki.jtcvm.2025.066
不同产地盒果藤中总黄酮提取工艺优化及含量测定
Extraction optimization and content determination of total flavonoids from Operculina turpethum(Linn.)S.Manso originating from different geographical sources
摘要
Abstract
The objective was to optimize the extraction protocol for total flavonoids from Operculina turpethum(Linn.)S.Manso,establish a reliable method for its content determination,and provide a robust technical basis for subsequent investigations of this species.Extraction yield of total flavonoids was employed as the sole evaluation criterion.Single-factor experiments were conducted to investigate the individual effects of ethanol concentration,liquid-solid ratio,and ultrasonic duration on the yield.On the basis of these results,L9(34)orthogonal array design was adopted to finalize the optimal conditions.The validated protocol was subsequently applied to quantify total flavonoids in O.turpethum samples collected from different geographical origins.The results showed that the optimal parameters were identified as 70%ethanol,a liquid-solid ratio of 15∶1(mL∶g),and an ultrasonic treatment time of 40 min.Under these conditions,the total flavonoid content in samples of varying provenance ranged from 0.68%to 1.79%,expressed as rutin equivalents.The optimized procedure demonstrates excellent repeatability and stability,thus providing a reliable method for routine extraction and quantification of total flavonoids from O.turpethum.Notably,flavonoid levels differ significantly among samples from different geographical origins,highlighting the importance of provenance-based quality control.关键词
盒果藤/总黄酮/提取工艺/含量测定Key words
Operculina turpethum(Linn.)S.Manso/total flavonoids/extraction technology/content determination分类
畜牧业引用本文复制引用
蒙玉珍,严秋婷,杨秉铮,黄颖,李兵..不同产地盒果藤中总黄酮提取工艺优化及含量测定[J].中兽医医药杂志,2025,44(6):58-62,5.基金项目
广西自然科学基金项目(2023GXNSFAA026362,2018GXNSFAA281138) （2023GXNSFAA026362,2018GXNSFAA281138）
广西中医药大学赛恩斯新医药学院科研项目(2023MS003) （2023MS003）
自治区级大学生创新训练计划项目(S202410600128) （S202410600128）
广西特色中药现代产业学院立项项目(C202405) （C202405）
广西中医药大学研究生教育创新计划项目(XYJ25093) （XYJ25093）
