中药与临床2025，Vol.16Issue(6)：18-23,27,7.
基于UPLC-Q-Orbitrap MS技术的小檗皮与余甘子配伍前后化学成分变化研究
Study on the changes of chemical components before and after the compatibility of Berberidis Cortex-Phyllanthi Fruc-tus based on UPLC-Q-Orbitrap MS technology
摘要
Abstract
Objective:To study the chemical composition changes of Tibetan medicine Berberidis Cortex and Phyllanthi Fructus before and after compatibility,and to explore the rationality of the compatibility of the two drugs.Methods:UPLC-Q-Or-bitrap MS was used to analyze the chemical constituents of the single decoction and combined decoction of Berberidis Cortex and Phyllanthi Fructus.The chemical constituents were identified by comparing with the ion fragment information of the reference substance,relevant literature and database.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to reveal the chemical constituents of the single decoction and combined decoction of the two drugs.Results:A total of 33 chemical constituents were identified including 12 alkaloids,11 polyphenols,6 organic acids,2 fla-vonoids and 2 sugars.Through PCA and OPLS-DA analysis,it was found that there was a significant difference between the single decoction and the combined decoction of the two drugs.A total of 10 different components were screened according to Variable Importance in Projection(VIP)>3 and t test.The relative contents of berberine,jatrorrhizine,palmatine,bufotenidine,corilagin,6-O-galloylglucose,quinic acid,oblongine and fructose were significantly increased in the combined decoction,while the relative content of trehalose was significantly decreased in the combined decoction.Conclusion:After the compatibility of Berberidis Cortex and Phyllanthi Fructus,the dissolution of various active components in the combined decoction could be promoted.These components may be the material basis for the synergistic treatment of diseases by the compatibility of Berberidis Cortex and Phyllanthi Fructus.关键词
小檗皮/余甘子/配伍/超高效液相色谱-四极杆-静电场轨道阱质谱/化学成分Key words
Berberidis Cortex/Phyllanthi Fructus/compatibility/UPLC-Q-Orbitrap MS/chemical composition分类
医药卫生引用本文复制引用
刘景烨,彭家艳,宗毅,丁银,苟晓玲,张静,范刚..基于UPLC-Q-Orbitrap MS技术的小檗皮与余甘子配伍前后化学成分变化研究[J].中药与临床,2025,16(6):18-23,27,7.基金项目
四川省重点研发项目(2022YFS0434) （2022YFS0434）
