中医药信息2025，Vol.42Issue(11)：16-19,4.DOI:10.19656/j.cnki.1002-2406.20251103
补中益气汤对胃癌乏氧微环境的调控作用研究
Study on the Regulatory Effect of Buzhong Yiqi Decoction on Hypoxic Microenvironment in Gastric Cancer
摘要
Abstract
Objective:To investigate the mechanism of Buzhong Yiqi Decoction in improving vascular hypoxic microenvironment by observing its effect on the expression of hypoxia-inducible factor-1α(HIF-1α)in a nude mouse model of gastric cancer(GC)with syndrome of deficiency of spleen qi.Methods:Fifty BALB/c-nu nude mice were randomly divided into negative control group,model group,model blank group,Buzhong Yiqi group,and Shenqi Fuzheng group,with 10 mice in each group.The negative control group was fed with standard rodent diet and purified water,while other groups were subjected to the vinegar administration method to establish syndrome of deficiency of spleen qi model,then subcutaneously implanted with SGC-7901 GC cells to create disease-syndrome combined models.Drugs were administered during the entire modeling period,the model group received standard rodent diet and purified water,the model blank group received 10 mL normal saline by gavage daily,the Buzhong Yiqi group received 0.66 g/kg Buzhong Yiqi Decoction by gavage daily,and the Shenqi Fuzheng group received 2 mL/kg Shenqi Fuzheng Injection by gavage daily.After 4 weeks of continuous drug administration,samples were collected.Microvessel density(MVD)was observed by hematoxylin-eosin(HE)staining,and HIF-1α expression was detected by Western blot.Results:At the end of modeling,MVD in the model group was significantly higher than that in the negative control group(P<0.05).After drug intervention,both Shenqi Fuzheng and Buzhong Yiqi groups showed significantly lower MVD than that in the model blank group(P<0.05),with Buzhong Yiqi group showing lower MVD than Shenqi Fuzheng group(P<0.05).HIF-1α content in the model group was significantly higher than that in the negative control group(P<0.05),while after the drug intervention,HIF-1α content in shengi Fuzheng and Buzhong Yiqi groups showed significantly lower than that in the model blank group(P<0.05).Conclusion:In GC hypoxic microenvironment,HIF-1α shows high expression.Buzhong Yiqi Decoction reduces HIF-1α expression and inhibits angiogenesis,thereby decreasing GC infiltration and metastasis.关键词
补中益气汤/胃癌/微血管密度/缺氧诱导因子-1αKey words
Buzhong Yiqi Decoction/Gastric cancer/Microvessel density/Hypoxia-inducible factor-1α引用本文复制引用
赵双梅,李慧臻,祁向争,杨岩..补中益气汤对胃癌乏氧微环境的调控作用研究[J].中医药信息,2025,42(11):16-19,4.基金项目
天津市卫生和计划生育委员会中医中西医结合科研项目(2017159) （2017159）
