安徽农业大学学报2025，Vol.52Issue(5)：819-829,11.DOI:10.13610/j.cnki.1672-352x.20251112.001
基因叠加对转Bt玉米中Bt蛋白表达及抗虫性的影响
Effects of gene stacking on the expression patterns of Bt proteins and the control to pests in transgenic Bt maize
摘要
Abstract
[Objective]This study aimed to explore the effect of gene stacking on the expression patterns of Bt proteins and the control to pests in transgenic Bt maize.[Method]A comparative analysis of Bt protein expres-sion patterns and insect resistance was conducted between monogenic Bt maize expressing only Cry1Ab(Bt-S-1)and stacked Bt maize expressing both Cry1Ab and Vip3Aa(Bt-P-1 and Bt-P-2)using ELISA and bioassay meth-ods.[Result]The temporal expression trends of the two types of Bt maize were similar.Specifically,the Bt pro-teins in the leaves,stems,and roots exhibited a gradual increase during the vegetative stage,followed by a gradual decrease during the reproductive growth stage.However,notable differences in Bt protein expression content were observed between the two types of Bt maize.The Cry1Ab content in Bt-S-1 was significantly higher than that in the other two Bt maize varieties,while the Cry1Ab content in Bt-P-1 was significantly higher than in Bt-P-2 which exhibited a higher Vip3Aa content.These findings suggested that gene stacking may influence the expres-sion levels of Bt proteins leading to an antagonistic effect between the expressions of the two proteins.Further-more,the toxicity of Cry1Ab and Vip3Aa proteins as well as their synergistic effects on the Ostrinia furnacalis and Helicoverpa armigera were analyzed,and the results showed that Cry1Ab was significantly more toxic to both pests compared to Vip3Aa,indicating that the enhancement of insecticidal activity by Vip3Aa on Cry1Ab was very limited.[Conclusion]Cry1Ab serves as the primary gene in Bt maize expressing both Cry1Ab and Vip3Aa for controlling of the O.furnacalis and H.armigera.The findings of this research provide a theoretical reference for the commercialization of Bt maize and the development of future resistance management strategies.关键词
Bt玉米/基因叠加/Bt蛋白/表达规律/抗虫性Key words
Bt maize/gene stacking/Bt protein/expression patterns/control to pests分类
农业科技引用本文复制引用
李颖,高美静,卢莉娜,仲建锋,张洁,张召贤,张志勇..基因叠加对转Bt玉米中Bt蛋白表达及抗虫性的影响[J].安徽农业大学学报,2025,52(5):819-829,11.基金项目
农业生物育种国家科技重大专项(2022ZD04020) （2022ZD04020）
