安徽农业大学学报2025，Vol.52Issue(5)：830-834,5.DOI:10.13610/j.cnki.1672-352x.20250916.001
不同虫态烟草粉螟和烟草甲的分子鉴定
Molecular identification of different life stages of tobacco moth(Ephestia elutella)and tobacco beetle(Lasioderma serricorne)
摘要
Abstract
[Objective]To facilitate the early identification of eggs,larvae,and pupae of the tobacco moth(Ephestia elutella)and the cigarette beetle(Lasioderma serricorne),this study employed DNA barcoding tech-nology to conduct molecular identification of different developmental stages of these two tobacco storage pests,aiming to provide early warning for the prevention and control of stored tobacco pests.[Method]DNA was ex-tracted from four life stages of the tobacco moth and the tobacco beetle,including eggs,larvae,pupae,and adults.Species-specific primers were designed for PCR amplification of the mitochondrial DNA Cytochrome oxidase I(COI)gene from each life stage of the two pests.Subsequently,sequencing,alignment,and analysis of the ampli-fied sequences were conducted.[Result]Specific bands of the same size were amplified from the four life stages of both pests,indicating that the COI gene barcode could be used to identify tobacco moth and the cigarette beetle at all life stages,and the accuracy of the identification results was not affected by the DNA samples from different life stages.[Conclusion]The precise identification of the tobacco moth and the cigarette beetle can be achieved within a short time by amplifying the COI genes of two pest species using PCR technology.关键词
烟草粉螟/烟草甲/细胞色素氧化酶I(COI)基因/分子鉴定Key words
tobacco moth/tobacco beetle/Cytochrome oxidase I(COI)gene/molecular identification分类
农业科技引用本文复制引用
姚洁,柴欣,黄衍章,蒋磊,江彤..不同虫态烟草粉螟和烟草甲的分子鉴定[J].安徽农业大学学报,2025,52(5):830-834,5.基金项目
安徽中药材种植联合研究中心科研项目(yjzx2023012) （yjzx2023012）
安徽高校自然科学研究重点项目(KJ2021A1427) （KJ2021A1427）
安徽中药材种植联合研究中心科研项目(yjzx2023013) （yjzx2023013）
贵州省烟草公司科技项目(2020XM09) （2020XM09）
