安徽农业科学2025，Vol.53Issue(22)：1-5,5.DOI:10.3969/j.issn.0517-6611.2025.22.001
腐食性昆虫单斑鼓翅蝇幼虫肠道共生菌多样性分析
Analysis of the Diversity of Symbiotic Bacteria in the Intestinal Tract of Larvae of the Saprophagous Insect,the Sepsis monostigma
摘要
Abstract
[Objective]To investigate the diversity of intestinal symbiotic bacteria in the larvae of the saprophagous insect Sepsis monostigma.[Method]The V3-V4 hypervariable regions of the 16S rRNA gene from intestinal symbiotic bacteria in third-instar larvae of S.monostigma were sequenced using Illumina MiSeq high-throughput sequencing technology to explore the community diversity,structure,and functional characteristics of the larval gut microbiota.[Result]A total of 547 896 valid sequences were obtained from five samples and clustered into 2 106 operational taxonomic units(OTUs).These OTUs were classified into 26 phyla,67 classes,168 orders,320 families,742 genera.The ACE index,Chao index,Shannon index,and Simpson index of the intestinal symbiotic bacterial community ranged from 691 to 787,673 to 751,2.83 to 3.52,and 0.089 5 to 0.231 7,respectively.Firmicutes and Proteobacteria were identified as the dominant phyla,while Providen-cia and Clostridium were the predominant genera.Functional prediction based on Tax4Fun revealed that the intestinal symbiotic bacteria were enriched in pathways related to ABC transporters,carbon metabolism,and fatty acid metabolism.[Conclusion]These findings provide valua-ble insights into the intestinal microbial resources of S.monostigma and offer a scientific reference for the application of this species in ecologi-cal environment purification.关键词
腐食性昆虫/单斑鼓翅蝇/高通量测序/肠道共生菌/多样性/菌群功能Key words
Saprophagous insect/Sepsis monostigma/High-throughput sequencing/Intestinal symbionts/Diversity/Microbiota function分类
农业科技引用本文复制引用
吴艳娜,王剑峰..腐食性昆虫单斑鼓翅蝇幼虫肠道共生菌多样性分析[J].安徽农业科学,2025,53(22):1-5,5.基金项目
科技部基础资源调查专项(2023FY100302). （2023FY100302）
