安徽农业科学2025，Vol.53Issue(22)：35-39,5.DOI:10.3969/j.issn.0517-6611.2025.22.008
菊花"淡妆垂露"茎尖脱毒快繁技术及规模化生产研究
Shoot Tip Detoxification Rapid Propagation Technology and Large-scale Production of Chrysanthemum"Danzhuangchuilu"
摘要
Abstract
Using the shoot tip growth point of chrysanthemum"Danzhuangchuilu"as the explant,in vitro tissue culture and formula optimiza-tion were carried out.The results showed that the optimal induction medium for adventitious bud of"Danzhuangchuilu"was MS+0.1 mg/L NAA+0.5 mg/L 6-BA+30 g/L sucrose+7 g/L agar.The optimal breeding medium for adventitious buds was MS+0.1 mg/L NAA+1.5 mg/L 6-BA+25 g/L sucrose+7 g/L agar.The multiplication coefficient reached 3.5.The optimal rooting medium was 1/2MS+0.1 mg/L NAA+15 g/L sucrose+7 g/L agar.The optimum substrate formula for transplanting was peat soil+vermiculite+perlite,the ratio was 3∶1∶0.5.An efficient shoot tip detoxification and rapid propagation system was finally developed.Combined with effective cultivation measures,the large-scale pro-duction of"Danzhuangchuilu"was realized,and a total of 20 000 plants were produced.The disease resistance of shoot tip detoxification seed-lings was better than that of traditional cuttings.关键词
菊花/淡妆垂露/茎尖脱毒/快繁体系Key words
Chrysanthemum/Danzhuangchuilu/Shoot tip detoxification/Rapid propagation system分类
农业科技引用本文复制引用
吴雯雯,罗茂婵,陆春,施霞,朱方丽,伍静..菊花"淡妆垂露"茎尖脱毒快繁技术及规模化生产研究[J].安徽农业科学,2025,53(22):35-39,5.基金项目
江苏省南通市社会民生科技计划项目(MSZ2023025). （MSZ2023025）
