川北医学院学报2025，Vol.40Issue(11)：1361-1364,1389,5.DOI:10.3969/j.issn.1005-3697.2025.11.001
基于TGF-β1/Smads信号通路探究灯盏花乙素对博来霉素诱导小鼠肺纤维化的改善机制
Exploration on the mechanism of scutellarin improving bleomycin-in-duced pulmonary fibrosis in mice based on TGF-β1/Smads signaling path-ways
摘要
Abstract
Objective:To explore the improvement effect of scutellarin(SCU)on bleomycin(BLM)-induced pulmonary fi-brosis in mice and to analyze potential action mechanism of SCU based on transforming growth factor-β1(TGF-β1)/Smads sig-naling pathways.Methods:The models of pulmonary fibrosis mice were constructed by intratracheal injection of BLM,and they were divided into blank group,SCU group,BLM group and BLM+SCU group.After 21 d of intervention,growth status,patho-logical changes of lung tissues,levels of inflammatory factors,expressions of pulmonary fibrosis markers proteins and mRNA levels of TGF-β1,Smad2,Smad3 and Smad7 in different groups were observed.Results:Compared with blank group and SCU group,scores of alveolar inflammation and pulmonary fibrosis,levels of interleukin-6(IL-6),IL-1β,tumor necrosis factor-α(TNF-α)and hydroxyproline(HYP),expressions of α-SMA,Collagen I and Fibronectin proteins in lung tissues,mRNA levels of TGF-β1 and Smad2/3/7 were increased in BLM group(P<0.05).The above indexes in BLM+SCU group were all lower than those in BLM group(P<0.05).Conclusion:SCU can improve BLM-induced pulmonary fibrosis and inflammatory response of lung tissues in mice.The improvement mechanism may be related to the down-regulation of TGF-β1/Smads pathways.关键词
肺纤维化/灯盏花乙素/转化生长因子-β1/Smads/炎症反应Key words
Pulmonary fibrosis/Scutellarin/Transformation growth factor-β1/Smads/Inflammatory response分类
医药卫生引用本文复制引用
丘圆圆,秦会平,王昌明,葛润,胡小文,陈小伊..基于TGF-β1/Smads信号通路探究灯盏花乙素对博来霉素诱导小鼠肺纤维化的改善机制[J].川北医学院学报,2025,40(11):1361-1364,1389,5.基金项目
国家自然科学基金地区科学基金项目(82160014 （）
82160002) （）
广西壮族自治区桂林市技术应用与推广计划(20210227-10-1) （20210227-10-1）
