川北医学院学报2025，Vol.40Issue(11)：1365-1370,6.DOI:10.3969/j.issn.1005-3697.2025.11.002
19个X-STRs基因座在四川少数民族人群中的法医学应用研究和群体结构分析
Forensic application research and population structure analysis of 19 X-STRs loci in Sichuan ethnic minority population
摘要
Abstract
Objective:To investigate the genetic polymorphism of 19 X-STRs loci in the Beichuan Qiang and Xichang Yi ethnic groups in Sichuan Province,evaluate their forensic application value,and analyze their population structure.Methods:Dry blood spot samples were collected from 246 unrelated individuals(188 males and 58 females)of the Beichuan Qiang ethnic group and 281 unrelated individuals(116 males and 165 females)of the Xichang Yi ethnic group.The dry blood spot samples were directly amplified using the MicroreaderTM19X ID System kit,and the PCR products were subjected to capillary electro-phoresis using an ABI3500 genetic analyzer,GeneMapper®ID-X software automatically classifies.Results:A total of 150 alleles were detected in the Beichuan Qiang ethnic group,with a frequency range of 0.003 3～0.828 9.In the Xichang Yi ethnic group,a total of 164 alleles were detected,with a frequency range of 0.002 2～0.800 4.After statistical testing,the gene frequency dis-tribution of the two ethnic minority groups conform to Hardy Weinberg equilibrium law(P>0.05),and after Bonferroni correc-tion,there were no loci with linkage disequilibrium.In the Beichuan Qiang ethnic group,the combined discrimination power for males(PDM)and combined discrimination power for females(PDF)were 0.999 999 999 945 624 and 0.999 999 999 999 999 993 22,re-spectively.The combined mean exclusion chance in duos(MECD)and mean exclusion chance in trios(MECT)were 0.999 999 591 813 975 and 0.999 999 999 465 296,respectively.In the Yi ethnic group of Xichang,the combined discrimination power for males(PDM)and combined discrimination power for females(PDF)were 0.999 999 999 873 529 and 1,respectively.The combined mean exclusion chance in duos(MECD)and mean exclusion chance in trios(MECT)were 0.999 999 284 224 924 and 0.999 999 998 931 424,respec-tively.Conclusion:The MicroreaderTM19X ID fluorescence detection kit has a high overall polymorphism and good discriminatory ability in the Beichuan Qiang and Xichang Yi populations,and can serve as a powerful supplement and verification method for parental relation-ships and individual identification.关键词
法医物证学/X-STR/群体遗传学/北川羌族/西昌彝族Key words
Forensic evidence science/X-STR/Population genetics/Beichuan Qiang/Xichang Yi分类
医药卫生引用本文复制引用
杨慧凌,郭天齐,李宇桐,范庆炜,徐冬冬,邓小冬,杜冰..19个X-STRs基因座在四川少数民族人群中的法医学应用研究和群体结构分析[J].川北医学院学报,2025,40(11):1365-1370,6.基金项目
四川省自然科学基金项目(2022NSFSC1286) （2022NSFSC1286）
川北医学院博士科研启动基金(CBY24-QDA14) （CBY24-QDA14）
川北医学院青年项目(CBY22-QNA25) （CBY22-QNA25）
