康复学报2025，Vol.35Issue(5)：494-503,10.DOI:10.3724/SP.J.1329.2025.05008
"形神共调"康复训练对认知衰弱小鼠脑区局部一致性的影响
Effect of"Mind-Body Co-Regulation"Rehabilitation Training on Regional Homogeneity of Brain in Mice with Cognitive Frailty
摘要
Abstract
Objective To explore the mechanism by which"Mind-Body Co-regulation"rehabilitation training improves mo-tor and cognitive functions in mice with cognitive frailty.Methods Six-month-old C57BL/6 mice served as the adult control group.Sixteen-month-old male C57BL/6 mice were randomly assigned to four groups(n=10 per group):cognitive frailty group,physical-tuning group,spirit-tuning group,and mind-body group.The physical-tuning group underwent treadmill training(passive motion at 12 m/min,1 hour/session,once daily).The spirit-tuning group was housed in a Shen-Nourishing Chamber(2 hours/ses-sion,once daily).The mind-body group received the same treadmill training as the physical-tuning group followed by Shen-Nourish-ing Chamber housing.The adult control group and cognitive frailty group were housed in standard cages without additional interven-tion.After 6 weeks of intervention,muscle strength was assessed using the grip strength test;balance ability was evaluated with the rotarod test.Hematoxylin and eosin(HE)staining and Masson's trichrome staining were employed to measure the cross-sectional area percentage of muscle fibers and collagen fiber deposition,respectively.Cognitive function was evaluated using the novel object recognition test and Morris water maze test.Subsequently,Bold-fMRI scanning was performed to analyze changes in regional homo-geneity.Results Compared with the adult control group,the muscle strength of the cognitive frailty group was significantly reduced(P<0.05);compared with the cognitive frailty group,the muscle strength of the physical-tuning group and the mind-body group was enhanced(P<0.05);compared with the spirit-tuning group,both the physical-tuning group and the mind-body group showed increased muscle strength(P<0.05).Compared with the adult control group,the cognitive frailty group showed a reduction in the rotating rod dwell time(P<0.05);compared with the cognitive frailty group,the physical-tuning group,the spirit-tuning group,and the mind-body group showed an increase in the rotating rod dwell time(P<0.05);compared with the spirit-tuning group,the physi-cal-tuning group and the mind-body group had a longer rotating rod dwell time(P<0.05);compared with the physical-tuning group,the mind-body group showed an increase in the rotating rod dwell time(P<0.05).Compared with the adult control group,the propor-tion of muscle fiber cross-sectional area decreased(P<0.05)and collagen fiber deposition increased(P<0.05)in the cognitive frailty group;compared with the cognitive frailty group,the proportion of muscle fiber cross-sectional area increased(P<0.05)and colla-gen fiber deposition decreased(P<0.05)in the physical-tuning group and the mind-body group;compared with the spirit-tuning group,the proportion of muscle fiber cross-sectional area increased(P<0.05)and collagen fiber deposition decreased(P<0.05)in the physical-tuning group and the mind-body group.Compared with the adult control group,the object recognition index of the cogni-tive frailty group decreased(P<0.05);compared with the cognitive frailty group,the object recognition index increased in the physi-cal-tuning group,spirit-tuning group,and mind-body group(P<0.05);compared with the physical-tuning group and the spirit-tuning group,the object recognition index of the mind-body group increased(P<0.05).Compared with the adult control group,the escape latency of the cognitive frailty group was prolonged from day 1 to day 4(P<0.05);compared with the cognitive frailty group,the escape latency of the physical-tuning group decreased on day 3 and day 4(P<0.05),and the escape latency of the mind-body group decreased from day 1 to day 4(P<0.05);compared with the physical-tuning group,the escape latency of the mind-body group de-creased on the third day(P<0.05).Compared with the adult control group,the cognitive frailty group showed a significant decrease in the number of platform crossings and the shorter duration in the target quadrant(P<0.05);compared with the cognitive frailty group,the physical-tuning group,the spirit-tuning group,and the mind-body group all showed an increased number of platform crossings and prolonged duration of stay in the target quadrant(P<0.05);compared with the physical-tuning and spirit-tuning groups,the mind-body group showed an increase in the number of platform crossings and longer duration of stay in the target quadrant(P<0.05).The ReHo analysis results showed that compared with the adult control group,the ReHo value in the right cortical amygdala area of the cognitive frailty group significantly increased(P<0.05);compared with the cognitive frailty group,the ReHo value in the right corti-cal amygdala region significantly decreased in the physical-tuning group,spirit-tuning group,and mind-body group(P<0.05).Con-clusion The mind-body co-regulation rehabilitation training directly or indirectly changes the functional activity of the amyg-dala through physical-tuning and spirit-tuning training,promoting the recovery of motor and cognitive abilities in mice with cogni-tive frailty.关键词
认知衰弱/认知功能/"形神共调"/杏仁核/局部一致性Key words
cognitive frailty/cognitive function/mind-body co-regulation/amygdala/regional homogeneity引用本文复制引用
王文举,林华伟,王思诺,梁胜祥,杨敏光,柳维林.."形神共调"康复训练对认知衰弱小鼠脑区局部一致性的影响[J].康复学报,2025,35(5):494-503,10.基金项目
国家自然科学基金面上项目(82274626) （82274626）
福建省卫健委中青年科研重大项目(2023ZQNZD015) （2023ZQNZD015）
福建省自然科学基金面上项目(2022J01377) （2022J01377）
福建中医药大学青年科研拔尖人才项目(XQB202203) （XQB202203）
