佛山科学技术学院学报（自然科学版）2025，Vol.43Issue(6)：89-96,8.
抗菌肽Protegrin-1对多重耐药性禽大肠杆菌的抑菌活性及稳定性研究
Study on the antibacterial activity and stability of the antimicrobial peptide Protegrin-1 against multidrug-resistant avian Escherichia coli
何胤霖 1徐静 1唐标 2陈胜锋 1刘璨颖1
作者信息
- 1. 佛山大学动物科技学院,广东 佛山 528225
- 2. 中国科学院大学杭州高等研究院生命与健康科学学院,浙江 杭州 310024
- 折叠
摘要
Abstract
To explore the potential of the antimicrobial peptide Protegrin-1(PG-1)as an alternative to antibiotics,this study investigated its bacteriostatic activity,biofilm formation-inhibiting ability,and bacteriostatic stability against two clinically isolated avian Escherichia coli(E.coli)strains,namely HNY006 and HNJ006.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of PG-1 against the two strains were determined by broth microdilution.The effect of PG-1 on biofilm formation was assessed by crystal violet staining.The inhibitory rate of PG-1 under different temperatures,pH levels,and UV treatment conditions was evaluated by spectrophotometry.The results showed that both the MIC and MBC of PG-1 against the two strains were 64 μg/mL,and PG-1 at the MIC concentration significantly inhibited biofilm formation of the two strains(P＜0.05).Additionally,PG-1 exhibited good thermal stability,acid-base stability,and UV stability.The above results indicate that PG-1 inhibits avian E.coli,suppresses its biofilm formation,and exhibits good stability.关键词
抗菌肽/禽大肠杆菌/生物被膜/抑菌活性Key words
antimicrobial peptides/avian Escherichia coli/biofilm/antimicrobial activity分类
农业科技引用本文复制引用
何胤霖,徐静,唐标,陈胜锋,刘璨颖..抗菌肽Protegrin-1对多重耐药性禽大肠杆菌的抑菌活性及稳定性研究[J].佛山科学技术学院学报（自然科学版）,2025,43(6):89-96,8.
